Doublecortin
HUABIO
catalog: ET1701-98

domestic rabbit monoclonal (JJ0959)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: Hippocampus Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

Western blot analysis of Doublecortin on different lysates with Rabbit anti-Doublecortin antibody (ET1701-98) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate (20 µg/Lane) Lane 2: Human brain tissue lysate (40 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 41 kDa Observed band size: 41/45 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-98) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Doublecortin
HUABIO
catalog: HA601398

domestic rabbit polyclonal (JJ0959)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rat anti-Doublecortin antibody (HA601398) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601398) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rat anti-Doublecortin antibody (HA601398) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601398) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier

Doublecortin
HUABIO
catalog: HA601495

domestic rabbit polyclonal (JJ0959)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Western blot analysis of Doublecortin on different lysates with Chicken anti-Doublecortin antibody (HA601495) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (15 µg/Lane) Lane 2: A549 cell lysate (negative) (15 µg/Lane) Lane 3: Mouse brain (P0) tissue lysate (20 µg/Lane) Lane 4: Mouse brain (P60) tissue lysate (20 µg/Lane) Lane 5: Mouse brain (P60) tissue lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 41/45 kDa Exposure time: 2 minutes 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601495) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit Anti-Chicken IgY - HRP Secondary Antibody (HA1026) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier

Doublecortin
HUABIO
catalog: HA601496

domestic rabbit polyclonal (JJ0959)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Western blot analysis of Doublecortin on different lysates with Guinea pig anti-Doublecortin antibody (HA601496) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (15 µg/Lane) Lane 2: A549 cell lysate (negative) (15 µg/Lane) Lane 3: Mouse brain (P0) tissue lysate (20 µg/Lane) Lane 4: Mouse brain (P60) tissue lysate (20 µg/Lane) Lane 5: Mouse brain (P60) tissue lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 41/45 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601496) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier

Doublecortin
HUABIO
catalog: HA601497

domestic rabbit polyclonal (JJ0959)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-Doublecortin antibody (HA601497) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601497) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Mouse anti-Doublecortin antibody (HA601497) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601497) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

Doublecortin
HUABIO
catalog: HA610238

domestic rabbit polyclonal (JJ0959)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rat anti-Doublecortin antibody (HA610238) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610238) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rat anti-Doublecortin antibody (HA610238) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610238) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier

Doublecortin
HUABIO
catalog: HA610325

domestic rabbit polyclonal (JJ0959)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Western blot analysis of Doublecortin on different lysates with Chicken anti-Doublecortin antibody (HA610325) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (15 µg/Lane) Lane 2: A549 cell lysate (negative) (15 µg/Lane) Lane 3: Mouse brain (P0) tissue lysate (20 µg/Lane) Lane 4: Mouse brain (P60) tissue lysate (20 µg/Lane) Lane 5: Mouse brain (P60) tissue lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 41/45 kDa Exposure time: 2 minutes 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610325) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit Anti-Chicken IgY - HRP Secondary Antibody (HA1026) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Doublecortin
HUABIO
catalog: HA610326

domestic rabbit polyclonal (JJ0959)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Western blot analysis of Doublecortin on different lysates with Guinea pig anti-Doublecortin antibody (HA610326) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (15 µg/Lane) Lane 2: A549 cell lysate (negative) (15 µg/Lane) Lane 3: Mouse brain (P0) tissue lysate (20 µg/Lane) Lane 4: Mouse brain (P60) tissue lysate (20 µg/Lane) Lane 5: Mouse brain (P60) tissue lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 41/45 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610326) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Doublecortin
HUABIO
catalog: HA610327

domestic rabbit polyclonal (JJ0959)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-Doublecortin antibody (HA610327) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610327) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Mouse anti-Doublecortin antibody (HA610327) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610327) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier