HUABIO Src antibody

Phospho-Src (Y419)
HUABIO
catalog: ET1609-15

domestic rabbit monoclonal (ST0800)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of Phospho-Src (Y419) on different lysates with Rabbit anti-Phospho-Src (Y419) antibody (ET1609-15) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-15) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Phospho-Src (Y419) on A431 cell lysates with Rabbit anti-Phospho-Src (Y419) antibody (ET1609-15) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-15) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of A549 cells labeling Phospho-Src (Y419) with Rabbit anti-Phospho-Src (Y419) antibody (ET1609-15) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Phospho-Src (Y419) antibody (ET1609-15) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
to the supplier

Src
HUABIO
catalog: ET1609-65

domestic rabbit monoclonal (ST05-03)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Src on different lysates with Rabbit anti-Src antibody (ET1609-65) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD Src cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 56 kDa Observed band size: 54 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-65) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Src on different lysates with Rabbit anti-Src antibody (ET1609-65) at 1/1,000 dilution. Lane 1: A549 cell lysate (10 µg/Lane) Lane 2: A431 cell lysate (10 µg/Lane) Lane 3: Mouse cerebellum tissue lysate (20 µg/Lane) Lane 4: Mouse lung tissue lysate (20 µg/Lane) Lane 5: Mouse liver tissue lysate (20 µg/Lane) Lane 6: Rat cerebellum tissue lysate (20 µg/Lane) Lane 7: Rat lung tissue lysate (20 µg/Lane) Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-65) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of A431 cells labeling Src with Rabbit anti-Src antibody (ET1609-65) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Src antibody (ET1609-65) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier

Src
HUABIO
catalog: ET1702-03

domestic rabbit monoclonal (JF0947)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Src on different lysates with Rabbit anti-Src antibody (ET1702-03) at 1/1,000 dilution. Lane 1: SK-OV-3 cell lysate, 20 µg/Lane Lane 2: A549 cell lysate, 20 µg/Lane Lane 3: NIH/3T3 cell lysate, 20 µg/Lane Lane 4: 4T1 cell lysate, 20 µg/Lane Lane 5: PC-12 cell lysate, 20 µg/Lane Lane 6: C6 cell lysate, 20 µg/Lane Lane 7: COS-1 cell lysate, 20 µg/Lane Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-03) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

All lanes: Western blot analysis of Src with anti-Src antibody [JF0947] (ET1702-03) at 1:1,000 dilution. Lane 1: Wild-type HCT116 whole cell lysate (20 µg). Lane 2: Src knockout HCT116 whole cell lysate (20 µg). ET1702-03 was shown to specifically react with Src in wild-type HCT116 cells. No band was observed when Src knockout sample was tested. Wild-type and Src knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-03, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of SK-OV-3 cells labeling Src with Rabbit anti-Src antibody (ET1702-03) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Src antibody (ET1702-03) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Src (S17)
HUABIO
catalog: HA723270

domestic rabbit monoclonal (PSH11-03)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Immunocytochemistry analysis of L6 cells untreated / treated with λpp labeling Phospho-Src (S17) with Rabbit anti-Phospho-Src (S17) antibody (HA723270) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Src (S17) antibody (HA723270) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of A549 cells labeling Phospho-Src (S17) with Rabbit anti-Phospho-Src (S17) antibody (HA723270) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Src (S17) antibody (HA723270) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Src (Y419)
HUABIO
catalog: HA750164

domestic rabbit monoclonal (ST0800)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of Phospho-Src (Y419) on different lysates with Rabbit anti-Phospho-Src (Y419) antibody (HA750164) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750164) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Phospho-Src (Y419) on A431 cell lysates with Rabbit anti-Phospho-Src (Y419) antibody (HA750164) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750164) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of A549 cells labeling Phospho-Src (Y419) with Rabbit anti-Phospho-Src (Y419) antibody (HA750164) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Phospho-Src (Y419) antibody (HA750164) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 649.00 USD
to the supplier

Src
HUABIO
catalog: HA750331

domestic rabbit monoclonal (JF0947)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Src on different lysates with Rabbit anti-Src antibody (HA750331) at 1/1,000 dilution. Lane 1: SK-OV-3 cell lysate, 20 µg/Lane Lane 2: A549 cell lysate, 20 µg/Lane Lane 3: NIH/3T3 cell lysate, 20 µg/Lane Lane 4: 4T1 cell lysate, 20 µg/Lane Lane 5: PC-12 cell lysate, 20 µg/Lane Lane 6: C6 cell lysate, 20 µg/Lane Lane 7: COS-1 cell lysate, 20 µg/Lane Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-03) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

All lanes: Western blot analysis of Src with anti-Src antibody [JF0947] (HA750331) at 1:1,000 dilution. Lane 1: Wild-type HCT116 whole cell lysate (20 µg). Lane 2: Src knockout HCT116 whole cell lysate (20 µg). ET1702-03 was shown to specifically react with Src in wild-type HCT116 cells. No band was observed when Src knockout sample was tested. Wild-type and Src knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-03, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of SK-OV-3 cells labeling Src with Rabbit anti-Src antibody (HA750331) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Src antibody (HA750331) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-Src (S17)
HUABIO
catalog: HA751393

domestic rabbit monoclonal (PSH11-03)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Immunocytochemistry analysis of L6 cells untreated / treated with λpp labeling Phospho-Src (S17) with Rabbit anti-Phospho-Src (S17) antibody (HA751393) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Src (S17) antibody (HA751393) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of A549 cells labeling Phospho-Src (S17) with Rabbit anti-Phospho-Src (S17) antibody (HA751393) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Src (S17) antibody (HA751393) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier

SRC
HUABIO
catalog: R1312-9

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Western blot analysis on cell lysates using anti-SRC rabbit polyclonal antibodies.

ICC staining SRC in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier