domestic rabbit monoclonal (SA93-03)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (ET1601-20) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution. Lane 1: MDA-MB-231 cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: K-562 cell lysate (15 µg/Lane) Lane 4: NIH/3T3 cell lysate (15 µg/Lane) Lane 5: PC-12 cell lysate (15 µg/Lane) Lane 6: Mouse kidney tissue lysate (15 µg/Lane) Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-20) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (ET1601-20) at 1/1,000 dilution. Lane 1: HEK293-si NT cell lysate (no heat) (10 µg/Lane) Lane 2/3: HEK293-si VDAC1 cell lysate (no heat) (10 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 17 seconds; ECL: K1801; 4-20% SDS-PAGE gel. ET1601-20 was shown to specifically react with VDAC1 in HEK293-si NT cells. Weakened band were observed when HEK293-si VDAC1 samples were tested. HEK293-si NT and HEK293-si VDAC1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1601-20, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at 4 ℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling VDAC1 with Rabbit anti-VDAC1 antibody (ET1601-20) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-VDAC1 antibody (ET1601-20) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-90)
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of VDAC1 / 2 / 3 on different lysates with Rabbit anti-VDAC1 / 2 / 3 antibody (HA723692) at 1/5,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse heart tissue lysate Lane 6: Rat brain tissue lysate Lane 7: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 35 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723692) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of VDAC1 / 2 / 3 on different lysates with Rabbit anti-VDAC1 / 2 / 3 antibody (HA723692) at 1/5,000 dilution. Lane 1: Human VDAC2 recombinant protein Lane 2: Human VDAC1 recombinant protein Lane 3: Human VDAC3 recombinant protein Lysates/proteins at 30 ng/Lane. Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723692) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (SA93-03)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (HA750012) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution. Lane 1: MDA-MB-231 cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: K-562 cell lysate (15 µg/Lane) Lane 4: NIH/3T3 cell lysate (15 µg/Lane) Lane 5: PC-12 cell lysate (15 µg/Lane) Lane 6: Mouse kidney tissue lysate (15 µg/Lane) Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750012) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (HA750012) at 1/1,000 dilution. Lane 1: HEK293-si NT cell lysate (no heat) (10 µg/Lane) Lane 2/3: HEK293-si VDAC1 cell lysate (no heat) (10 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 17 seconds; ECL: K1801; 4-20% SDS-PAGE gel. ET1601-20 was shown to specifically react with VDAC1 in HEK293-si NT cells. Weakened band were observed when HEK293-si VDAC1 samples were tested. HEK293-si NT and HEK293-si VDAC1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1601-20, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at 4 ℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling VDAC1 with Rabbit anti-VDAC1 antibody (HA750012) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-VDAC1 antibody (HA750012) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-90)
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of VDAC1 / 2 / 3 on different lysates with Rabbit anti-VDAC1 / 2 / 3 antibody (HA751534) at 1/5,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse heart tissue lysate Lane 6: Rat brain tissue lysate Lane 7: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 35 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751534) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of VDAC1 / 2 / 3 on different lysates with Rabbit anti-VDAC1 / 2 / 3 antibody (HA751534) at 1/5,000 dilution. Lane 1: Human VDAC2 recombinant protein Lane 2: Human VDAC1 recombinant protein Lane 3: Human VDAC3 recombinant protein Lysates/proteins at 30 ng/Lane. Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751534) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (R1307-1) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: A431 cell lysate Lane 4: Raji cell lysate Lane 5: HepG2 cell lysate Lane 6: SW480 cell lysate Lane 7: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-1) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (R1307-1) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: C2C12 cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: Mouse skeletal muscle tissue lysate (40 µg/Lane) Lane 5: Rat skeletal muscle tissue lysate (40 µg/Lane) Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-1) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (R1307-1) at 1/1,000 dilution. Lane 1: HEK293-si NT cell lysate (10 µg/Lane) Lane 2: HEK293-si VDAC1#1(no heat) cell lysate (10 µg/Lane) Lane 3: HEK293-si VDAC1#2(no heat) cell lysate (10 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. R1307-1 was shown to specifically react with VDAC1 in HEK293-si NT cells. No band were observed when HEK293-si VDAC1 samples were tested. HEK293-si NT and HEK293-si VDAC1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1307-1, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier