HUABIO presenilin 2 antibody

PEN2
HUABIO
catalog: ET7109-26

domestic rabbit monoclonal (JE41-28)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of PEN2 on mouse spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-26, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

ICC staining of PEN2 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of PEN2 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier

Presenilin 2 / AD5
HUABIO
catalog: HA722243

domestic rabbit monoclonal (PSH05-18)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation

Presenilin 2 / AD5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722243 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722243 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA722243 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA722243 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 59 seconds; ECL: K1801
quantity: 100μl
price: 385.00 USD
to the supplier

Presenilin 2 / AD5
HUABIO
catalog: HA72$3300

domestic rabbit monoclonal (PSH11-18)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry

quantity: 100μl
price: 385.00 USD
to the supplier

Presenilin 2 / AD5
HUABIO
catalog: HA750989

domestic rabbit monoclonal (PSH05-18)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation

Presenilin 2 / AD5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA750989 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750989 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA750989 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA750989 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 59 seconds; ECL: K1801
quantity: 100μl
price: 649.00 USD
to the supplier

Presenilin 2 / AD5
HUABIO
catalog: HA751399

domestic rabbit monoclonal (PSH11-18)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry

Western blot analysis of Presenilin 2 / AD5 on different lysates with Rabbit anti-Presenilin 2 / AD5 antibody (HA751399) at 1/2,000 dilution. Lane 1: HeLa cell lysate (no heat) Lane 2: SH-SY5Y cell lysate (no heat) Lane 3: RAW264.7 cell lysate (no heat) Lane 4: Neuro-2a cell lysate (no heat) Lane 5: PC-12 cell lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 23 kDa Exposure time: Lane 1-4: 3 minutes; Lane 3: 1 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751399) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of PC-12 cells labeling Presenilin 2 / AD5 with Rabbit anti-Presenilin 2 / AD5 antibody (HA751399) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Presenilin 2 / AD5 antibody (HA751399) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of PC-12 cells labeling Presenilin 2 / AD5. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751399, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier