domestic rabbit monoclonal (PSH03-87)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate (hot lysis) Lane 3: Rat brain tissue lysate (no heat) Lane 4: Mouse cerebellum tissue lysate (70℃ heat) Lane 5: Rat cerebellum tissue lysate Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative) Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative) Notice: no heat means the lysate is not boiled. Lysates/proteins at 40 µg/Lane. Predicted band size: 108 kDa Observed band size: 95-108 kDa Exposure time: Lane 1-7 (left): 28 seconds; Lane 1-5 (right): 2 minutes 34 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722053) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABA B Receptor 1 with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722053, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (HA722053) at 1/5,000 dilution. Lane 1: Human brain tissue lysate (40 µg/Lane) Lane 2: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 3: Rat brain tissue lysate (no heat) (20 µg/Lane) Lane 4: Mouse cerebellum tissue lysate (70℃ heat) (20 µg/Lane) Lane 5: Rat cerebellum tissue lysate (20 µg/Lane) Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane) Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 108 kDa Observed band size: 95-108 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722053) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH06-21)
reactivity: human, mouse, rat
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
Application: IHC-Fr Species: Mouse Site: Liver (Negative) Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH03-87)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (HA750912) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate (hot lysis) Lane 3: Rat brain tissue lysate (no heat) Lane 4: Mouse cerebellum tissue lysate (70℃ heat) Lane 5: Rat cerebellum tissue lysate Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative) Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative) Notice: no heat means the lysate is not boiled. Lysates/proteins at 40 µg/Lane. Predicted band size: 108 kDa Observed band size: 95-108 kDa Exposure time: Lane 1-7 (left): 28 seconds; Lane 1-5 (right): 2 minutes 34 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750912) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABA B Receptor 1 with Rabbit anti-GABA B Receptor 1 antibody (HA750912) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750912, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (HA750912) at 1/5,000 dilution. Lane 1: Human brain tissue lysate (40 µg/Lane) Lane 2: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 3: Rat brain tissue lysate (no heat) (20 µg/Lane) Lane 4: Mouse cerebellum tissue lysate (70℃ heat) (20 µg/Lane) Lane 5: Rat cerebellum tissue lysate (20 µg/Lane) Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane) Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 108 kDa Observed band size: 95-108 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750912) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH06-21)
reactivity: human, mouse, rat
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
Application: IHC-Fr Species: Mouse Site: Liver (Negative) Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
quantity: 100μl
price: 649.00 USD
to the supplier