domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of Lyn on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500142, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate Lane 2: A431 cell lysate Lane 2: Mouse liver tissue lysate
Western blot analysis of Lyn on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500142, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit monoclonal (JE44-75)
reactivity: human, mouse, rat
application: western blot, flow cytometry
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of Lyn on different lysates with Rabbit anti-Lyn antibody (HA721991) at 1/1,000 dilution. Lane 1: Ramos cell lysate (15 µg/Lane) Lane 2: A431 cell lysate (15 µg/Lane) Lane 3: Jurkat cell lysate (15 µg/Lane) Lane 4: THP-1 cell lysate (15 µg/Lane) Lane 5: Raji cell lysate (15 µg/Lane) Lane 6: K-562 cell lysate (15 µg/Lane) Lane 7: U-937 cell lysate (15 µg/Lane) Lane 8: Mouse lung tissue lysate (30 µg/Lane) Lane 9: Rat lung tissue lysate (30 µg/Lane) Predicted band size: 59 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721991) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Lyn on different lysates with Rabbit anti-Lyn antibody (HA721991) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si Lyn cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 59 kDa Observed band size: 55 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721991) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of A431 cells labeling Lyn. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721991, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH09-43)
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) on different lysates with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody (HA723105) at 1/2,000 dilution. Lane 1: COLO205 cell lysate Lane 2: TT cell lysate (negative) Lane 3: Ramos serum starved for 16 hours cell lysate Lane 4: Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate Lane 5: Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 23 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723105) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) on different lysates with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody (HA723105) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 treated with 50mM sodium orthovanadate for 5 minutes cell lysate (20 µg/Lane) Lane 3: PC-12 cell lysate (20 µg/Lane) Lane 4: PC-12 treated with 1mM sodium orthovanadate for 30 minutes cell lysate (20 µg/Lane) Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: Lane 1-2: 20 seconds; Lane 3-4: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723105) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Ramos cells serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes labeling Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody (HA723105) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody (HA723105) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH09-43)
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) on different lysates with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody (HA751290) at 1/2,000 dilution. Lane 1: COLO205 cell lysate Lane 2: TT cell lysate (negative) Lane 3: Ramos serum starved for 16 hours cell lysate Lane 4: Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate Lane 5: Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 23 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751290) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) on different lysates with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody (HA751290) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 treated with 50mM sodium orthovanadate for 5 minutes cell lysate (20 µg/Lane) Lane 3: PC-12 cell lysate (20 µg/Lane) Lane 4: PC-12 treated with 1mM sodium orthovanadate for 30 minutes cell lysate (20 µg/Lane) Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: Lane 1-2: 20 seconds; Lane 3-4: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751290) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Ramos cells serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes labeling Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody (HA751290) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody (HA751290) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier