domestic rabbit monoclonal (PSH08-46)
reactivity: mouse
conjugate: FITC
application: flow cytometry
reactivity: mouse
conjugate: FITC
application: flow cytometry
Flow cytometric analysis of C57BL/6 mouse spleen cells labeling NK1.1 (HA720219F, FITC). Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with NK1.1 (HA720219F, FITC, 1/1,000) compared with Rabbit IgG Isotype Control (FITC, 1/1,000).
quantity: 100μl
price: 429.00 USD
to the supplier
domestic rabbit monoclonal (PSH08-46)
reactivity: mouse
application: flow cytometry
reactivity: mouse
application: flow cytometry
Flow cytometric analysis of C57BL/6 mouse spleen cells labeling NK1.1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722992, 1/400). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 594 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1122) at 1/1,000 dilution for 30 minutes at +4℃.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH08-47)
reactivity: mouse
application: western blot, immunohistochemistry - paraffin section
reactivity: mouse
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of NK1.1 on NK1.1 recombinant protein with Rabbit anti-NK1.1 antibody (HA722993) at 1/1,000 dilution. Lysates/proteins at 50 ng/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 11 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722993) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded C57BL/6 mouse thymus tissue with Rabbit anti-NK1.1 antibody (HA722993) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722993) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH08-46)
reactivity: mouse
application: flow cytometry
reactivity: mouse
application: flow cytometry
Flow cytometric analysis of C57BL/6 mouse spleen cells labeling NK1.1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751221, 1/400). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 594 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1122) at 1/1,000 dilution for 30 minutes at +4℃.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH08-47)
reactivity: mouse
application: western blot, immunohistochemistry - paraffin section
reactivity: mouse
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of NK1.1 on NK1.1 recombinant protein with Rabbit anti-NK1.1 antibody (HA751222) at 1/1,000 dilution. Lysates/proteins at 50 ng/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 11 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751222) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded C57BL/6 mouse thymus tissue with Rabbit anti-NK1.1 antibody (HA751222) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751222) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier