HUABIO B7 H3 antibody

CD276
HUABIO
catalog: ER1803-36

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of CD276 on different lysates with Rabbit anti-CD276 antibody (ER1803-36) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HEK-293 cell lysate Lane 3: LoVo cell lysate Lane 4: U-2 OS cell lysate Lane 5: LNCaP cell lysate Lane 6: SH-SY5Y cell lysate Lane 7: THP-1 cell lysate Lane 8: HCT 116 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 57 kDa Observed band size: 100 kDa Exposure time: 42 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-36) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD276 on different lysates with Rabbit anti-CD276 antibody (ER1803-36) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-CD276 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 57 kDa Observed band size: 100 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-36) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of MCF7 cells labeling CD276 with Rabbit anti-CD276 antibody (ER1803-36) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD276 antibody (ER1803-36) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 330 USD
to the supplier

CD276
HUABIO
catalog: HA721245

domestic rabbit monoclonal (PD00-36)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD276 on different lysates with Rabbit anti-CD276 antibody (HA721245) at 1/5,000 dilution. Lane 1: HS-SY-II-si NT cell lysate Lane 2: HS-SY-II-si CD276 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 57 kDa Observed band size: 90 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721245) at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue with Rabbit anti-CD276 antibody (HA721245) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721245) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

CD276
HUABIO
catalog: HA721823

domestic rabbit monoclonal (PSH02-46)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of CD276 on different lysates with Rabbit anti-CD276 antibody (HA721823) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: LoVo cell lysate Lane 5: U-2 OS cell lysate Lane 6: LNCaP cell lysate Lane 7: SH-SY5Y cell lysate Lane 8: THP-1 cell lysate Lane 9: HCT 116 cell lysate Lane 10: Raji cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 57 kDa Observed band size: 100 kDa Exposure time: 42 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721823) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD276 on different lysates with Rabbit anti-CD276 antibody (HA721823) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate (10 µg/Lane) Lane 2: HAP1-CD276 KD cell lysate (10 µg/Lane) Predicted band size: 57 kDa Observed band size: 100 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721823) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of THP-1 cells labeling CD276 with Rabbit anti-CD276 antibody (HA721823) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD276 antibody (HA721823) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier

Human CD276
HUABIO
catalog: HA722491

domestic rabbit monoclonal (PSH05-92)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human CD276 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722491) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD276 protein (HA210964) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA722491, Biotin, 0.1 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native B7-H3 in human serum and human urine samples. The concentrations of B7-H3 were measured in triplicates, interpolated from the B7-H3 standard curve and corrected for sample dilution. Undiluted samples are human serum 5% and human urine 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean B7-H3 concentration was determined to be 19.1ng/ml in human serum and 4.9 ng/ml in human urine.

Interpolated concentrations of native B7-H3 in A431 and NIH:OVCAR-3 cell culture supernatant. The concentrations of B7-H3 were measured in triplicates, interpolated from the B7-H3 standard curve and corrected for sample dilution. Undiluted samples are A431 cell culture supernatant 100% and NIH:OVCAR-3 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean B7-H3 concentration was determined to be 199.5 pg/ml in A431 cell culture supernatant and 476.8 pg/ml in NIH:OVCAR-3 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Human CD276
HUABIO
catalog: HA722492

domestic rabbit monoclonal (PSH05-93)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human CD276 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722491) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD276 protein (HA210964) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA722491, Biotin, 0.1 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native B7-H3 in human serum and human urine samples. The concentrations of B7-H3 were measured in triplicates, interpolated from the B7-H3 standard curve and corrected for sample dilution. Undiluted samples are human serum 5% and human urine 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean B7-H3 concentration was determined to be 19.1ng/ml in human serum and 4.9 ng/ml in human urine.

Interpolated concentrations of native B7-H3 in A431 and NIH:OVCAR-3 cell culture supernatant. The concentrations of B7-H3 were measured in triplicates, interpolated from the B7-H3 standard curve and corrected for sample dilution. Undiluted samples are A431 cell culture supernatant 100% and NIH:OVCAR-3 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean B7-H3 concentration was determined to be 199.5 pg/ml in A431 cell culture supernatant and 476.8 pg/ml in NIH:OVCAR-3 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Human CD276
HUABIO
catalog: HA722588B

domestic rabbit monoclonal (PSH05-93)
reactivity: human
conjugate: biotin
application: ELISA

quantity: 100μl
price: 409.00 USD
to the supplier

CD276
HUABIO
catalog: HA750811

domestic rabbit monoclonal (PSH02-46)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of CD276 on different lysates with Rabbit anti-CD276 antibody (HA750811) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: LoVo cell lysate Lane 5: U-2 OS cell lysate Lane 6: LNCaP cell lysate Lane 7: SH-SY5Y cell lysate Lane 8: THP-1 cell lysate Lane 9: HCT 116 cell lysate Lane 10: Raji cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 57 kDa Observed band size: 100 kDa Exposure time: 42 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750811) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD276 on different lysates with Rabbit anti-CD276 antibody (HA750811) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate (10 µg/Lane) Lane 2: HAP1-CD276 KD cell lysate (10 µg/Lane) Predicted band size: 57 kDa Observed band size: 100 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750811) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of THP-1 cells labeling CD276 with Rabbit anti-CD276 antibody (HA750811) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD276 antibody (HA750811) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier