PD-L2
HUABIO
catalog: HA601331

mouse monoclonal (A10D8)
reactivity: human
application: western blot

Western blot analysis of PD-L2 on different lysates with Mouse anti-PD-L2 antibody (HA601331) at 1/1,000 dilution. Lane 1: Saos-2 cell lysate (hot lysis) Lane 2: Jurkat cell lysate (negative) Lane 3: MG-63 cell lysate (hot lysis) Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 50 kDa Exposure time: Lane 1-2: 1 minute; Lane 3: 2 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601331) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HDLM-2 (positive) and Jurkat (negative) labeling PD-L2 with Mouse anti-PD-L2 antibody (HA601331) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PD-L2 antibody (HA601331) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 360.00 USD
to the supplier

Human PD-L2
HUABIO
catalog: HA723145

domestic rabbit monoclonal (PSH09-80)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human PD-L2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723145) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Human PD-L2 (HA210599) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723147, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native PD-L2 in human urine, human serum samples and Jurkat cell culture supernatant. The concentrations of PD-L2 were measured in duplicate, interpolated from the PD-L2 standard curve and corrected for sample dilution. Undiluted samples are human urine 100%, human serum 40%, and Jurkat cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L2 concentration was determined to be 4,248 pg/ml in human urine, 10,104 pg/ml in human serum and undetectable in Jurkat cell culture supernatant.

Interpolated concentrations of spiked PD-L2 in human cell culture media samples. The concentrations of PD-L2 were measured in duplicates, interpolated from the PD-L2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Human PD-L2
HUABIO
catalog: HA723146

domestic rabbit monoclonal (PSH09-81)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human PD-L2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723146) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Human PD-L2 (HA210599) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723147, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native PD-L2 in human urine, human serum samples and Jurkat cell culture supernatant. The concentrations of PD-L2 were interpolated from the PD-L2 standard curve and corrected for sample dilution. Undiluted samples are human urine 100%, human serum 40%, and Jurkat cell culture supernatant 100%. The mean PD-L2 concentration was determined to be 4,983 pg/ml in human urine, 14,438 pg/ml in human serum and undetectable in Jurkat cell culture supernatant.

Interpolated concentrations of spiked PD-L2 in human cell culture media samples. The concentrations of PD-L2 were measured in duplicates, interpolated from the PD-L2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Human PD-L2
HUABIO
catalog: HA723147

domestic rabbit monoclonal (PSH09-82)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human PD-L2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723145) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PD-L2 (HA210599) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723147, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human PD-L2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723146) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PD-L2 (HA210599) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723147, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native PD-L2 in human urine, human serum samples and Jurkat cell culture supernatant. The concentrations of PD-L2 were measured in duplicate, interpolated from the PD-L2 standard curve and corrected for sample dilution. Undiluted samples are human urine 100%, human serum 40%, and Jurkat cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L2 concentration was determined to be 4,248 pg/ml in human urine, 10,104 pg/ml in human serum and undetectable in Jurkat cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Human PD-L2
HUABIO
catalog: HA723148B

domestic rabbit monoclonal (PSH09-82)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human PD-L2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723145) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Human PD-L2 (HA210599) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723148B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human PD-L2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723146) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Human PD-L2 (HA210599) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723148B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native PD-L2 in human urine, human serum samples and Jurkat cell culture supernatant. The concentrations of PD-L2 were measured in duplicate, interpolated from the PD-L2 standard curve and corrected for sample dilution. Undiluted samples are human urine 100%, human serum 40%, and Jurkat cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L2 concentration was determined to be 4,248 pg/ml in human urine, 10,104 pg/ml in human serum and undetectable in Jurkat cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier

Human PD-L2
HUABIO
catalog: HA723373H

domestic rabbit monoclonal (PSH09-82)
reactivity: human
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of Human PD-L2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723146) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PD-L2 (HA210599) starting from 111.11 ng/ml to 0 ng/ml and detect antibody (HA723373H, HRP, 0.01 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier