ULBP1
HUABIO
catalog: HA720092

domestic rabbit monoclonal (JE65-75)
reactivity: human
application: western blot

Western blot analysis of ULBP1 on SiHa cell lysates with Rabbit anti-ULBP1 antibody (HA720092) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720092) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Western blot analysis of ULBP1 on Jurkat cell lysates with Rabbit anti-ULBP1 antibody (HA720092) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720092) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Human ULBP1
HUABIO
catalog: HA723406

domestic rabbit monoclonal (PSH11-95)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human ULBP1 matched pair antibodies Capture: HA723406, Human ULBP1 Rabbit mAb [PSH11-95] Detector: HA723407, Human ULBP1 Rabbit mAb [PSH11-96] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723406) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ULBP1 protein (HA210963) starting from 2500 pg/ml to 0 pg/ml and detect antibody (HA723407, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of human ULBP1 matched pair antibodies Capture: HA723406, Human ULBP1 Rabbit mAb [PSH11-95] Detector: HA723409, Human ULBP1 Rabbit mAb [PSH11-97] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723406) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ULBP1 protein (HA210963) starting from 2500 pg/ml to 0 pg/ml and detect antibody (HA723409, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native ULBP1 in human cell culture supernatant samples. Capture: HA723406, Human ULBP1 Rabbit mAb [PSH11-95] Detector: HA723407, Human ULBP1 Rabbit mAb [PSH11-96] Interpolated concentration of native ULBP1 was measured in duplicate at different sample concentrations. Undiluted samples were 50% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ULBP1 concentration was determined to be 831 pg/mL in K562 cell supernatant. There was no detectable signal in HaCaT cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Human ULBP1
HUABIO
catalog: HA723407

domestic rabbit monoclonal (PSH11-96)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human ULBP1 matched pair antibodies Capture: HA723406, Human ULBP1 Rabbit mAb [PSH11-95] Detector: HA723407, Human ULBP1 Rabbit mAb [PSH11-96] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723406) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ULBP1 protein (HA210963) starting from 2500 pg/ml to 0 pg/ml and detect antibody (HA723407, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native ULBP1 in human cell culture supernatant samples. Capture: HA723406, Human ULBP1 Rabbit mAb [PSH11-95] Detector: HA723407, Human ULBP1 Rabbit mAb [PSH11-96] Interpolated concentration of native ULBP1 was measured in duplicate at different sample concentrations. Undiluted samples were 50% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ULBP1 concentration was determined to be 831 pg/mL in K562 cell supernatant. There was no detectable signal in HaCaT cell culture supernatant.

Interpolated concentrations of spiked ULBP1 in cell culture media samples. Capture: HA723406, Human ULBP1 Rabbit mAb [PSH11-95] Detector: HA723407, Human ULBP1 Rabbit mAb [PSH11-96] The concentrations of ULBP1 were measured in duplicates, interpolated from the ULBP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Human ULBP1
HUABIO
catalog: HA723408B

domestic rabbit monoclonal (PSH11-96)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human ULBP1 matched pair antibodies Capture: HA723406, Human ULBP1 Rabbit mAb [PSH11-95] Detector: HA723407, Human ULBP1 Rabbit mAb [PSH11-96] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723406) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ULBP1 protein (HA210963) starting from 2500 pg/ml to 0 pg/ml and detect antibody (HA723408B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native ULBP1 in human cell culture supernatant samples. Capture: HA723406, Human ULBP1 Rabbit mAb [PSH11-95] Detector: HA723407, Human ULBP1 Rabbit mAb [PSH11-96] Interpolated concentration of native ULBP1 was measured in duplicate at different sample concentrations. Undiluted samples were 50% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ULBP1 concentration was determined to be 831 pg/mL in K562 cell supernatant. There was no detectable signal in HaCaT cell culture supernatant.

Interpolated concentrations of spiked ULBP1 in cell culture media samples. Capture: HA723406, Human ULBP1 Rabbit mAb [PSH11-95] Detector: HA723407, Human ULBP1 Rabbit mAb [PSH11-96] The concentrations of ULBP1 were measured in duplicates, interpolated from the ULBP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier

Human ULBP1
HUABIO
catalog: HA723409

domestic rabbit monoclonal (PSH11-97)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human ULBP1 matched pair antibodies Capture: HA723406, Human ULBP1 Rabbit mAb [PSH11-95] Detector: HA723409, Human ULBP1 Rabbit mAb [PSH11-97] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723406) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ULBP1 protein (HA210963) starting from 2500 pg/ml to 0 pg/ml and detect antibody (HA723409, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native ULBP1 in human cell culture supernatant samples. Capture: HA723406, Human ULBP1 Rabbit mAb [PSH11-95] Detector: HA723409, Human ULBP1 Rabbit mAb [PSH11-97] Interpolated concentration of native ULBP1 was measured in duplicate at different sample concentrations. Undiluted samples were 50% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ULBP1 concentration was determined to be 767 pg/mL in K562 cell supernatant. There was no detectable signal in HaCaT cell culture supernatant.

Interpolated concentrations of spiked ULBP1 in cell culture media samples. Capture: HA723406, Human ULBP1 Rabbit mAb [PSH11-95] Detector: HA723409, Human ULBP1 Rabbit mAb [PSH11-97] The concentrations of ULBP1 were measured in duplicates, interpolated from the ULBP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Human ULBP1
HUABIO
catalog: HA723410B

domestic rabbit monoclonal (PSH11-97)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human ULBP1 matched pair antibodies Capture: HA723406, Human ULBP1 Rabbit mAb [PSH11-95] Detector: HA723409, Human ULBP1 Rabbit mAb [PSH11-97] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723406) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ULBP1 protein (HA210963) starting from 2500 pg/ml to 0 pg/ml and detect antibody (HA723410B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native ULBP1 in human cell culture supernatant samples. Capture: HA723406, Human ULBP1 Rabbit mAb [PSH11-95] Detector: HA723409, Human ULBP1 Rabbit mAb [PSH11-97] Interpolated concentration of native ULBP1 was measured in duplicate at different sample concentrations. Undiluted samples were 50% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ULBP1 concentration was determined to be 767 pg/mL in K562 cell supernatant. There was no detectable signal in HaCaT cell culture supernatant.

Interpolated concentrations of spiked ULBP1 in cell culture media samples. Capture: HA723406, Human ULBP1 Rabbit mAb [PSH11-95] Detector: HA723409, Human ULBP1 Rabbit mAb [PSH11-97] The concentrations of ULBP1 were measured in duplicates, interpolated from the ULBP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier