Gasdermin D (N terminal)
HUABIO
catalog: ER1901-37

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Gasdermin D (N terminal) on different lysates with Rabbit anti-Gasdermin D (N terminal) antibody (ER1901-37) at 1/1,000 dilution. Lane 1: SiHa cell lysate (10 µg/Lane) Lane 2: PC-3 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: THP-1 cell lysate (10 µg/Lane) Lane 5: Human kidney tissue lysate (20 µg/Lane) Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-37) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Gasdermin D (N terminal) on THP-1 cell lysates with Rabbit anti-Gasdermin D (N terminal) antibody (ER1901-37) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53/35 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-Gasdermin D (N terminal) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-37, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier

GSDMD
HUABIO
catalog: HA601046

mouse monoclonal (A8D7)
reactivity: human, mouse
application: western blot

Western blot analysis of GSDMD on different lysates with Mouse anti-GSDMD antibody (HA601046) at 1/500 dilution. Lane 1: PC-3 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601046) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier

GSDMD
HUABIO
catalog: HA601047

mouse monoclonal (A8D8)
reactivity: human
application: western blot

Western blot analysis of GSDMD on different lysates with Mouse anti-GSDMD antibody (HA601047) at 1/500 dilution. Lane 1: PC-3 cell lysate Lane 2: SiHa cell lysate Lane 3: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601047) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier

Gasdermin D (N terminal)
HUABIO
catalog: HA721144-50UL

domestic rabbit monoclonal (PD00-18)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Gasdermin D (N terminal) on different lysates with Rabbit anti-Gasdermin D (N terminal) antibody (HA721144) at 1/2,000 dilution. Lane 1: SiHa cell lysate Lane 2: Jurkat cell lysate Lane 3: THP-1 cell lysate Lane 4: PC-3M cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: L6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53/30 kDa Observed band size: 53/30 kDa Exposure time: 43 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721144) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Gasdermin D (N terminal) on different lysates with Rabbit anti-Gasdermin D (N terminal) antibody (HA721144) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate (20 µg/Lane) Predicted band size: 53/30 kDa Observed band size: 53/35 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721144) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse intestine tissue with Rabbit anti-Gasdermin D (N terminal) antibody (HA721144) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721144) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 50μl
price: 205.00 USD
to the supplier

Gasdermin D (N terminal)
HUABIO
catalog: HA723254

domestic rabbit monoclonal (PSH10-87)
reactivity: human
application: western blot

Western blot analysis of Gasdermin D (N terminal) on different lysates with Rabbit anti-Gasdermin D (N terminal) antibody (HA723254) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 35 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723254) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Gasdermin D (N terminal)
HUABIO
catalog: HA750486

domestic rabbit monoclonal (PD00-18)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Gasdermin D (N terminal) on different lysates with Rabbit anti-Gasdermin D (N terminal) antibody (HA750486) at 1/2,000 dilution. Lane 1: SiHa cell lysate Lane 2: Jurkat cell lysate Lane 3: THP-1 cell lysate Lane 4: PC-3M cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: L6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53/30 kDa Observed band size: 53/30 kDa Exposure time: 43 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750486) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Gasdermin D (N terminal) on different lysates with Rabbit anti-Gasdermin D (N terminal) antibody (HA750486) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate (20 µg/Lane) Predicted band size: 53/30 kDa Observed band size: 53/35 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750486) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse intestine tissue with Rabbit anti-Gasdermin D (N terminal) antibody (HA750486) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750486) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

Gasdermin D (N terminal)
HUABIO
catalog: HA751379

domestic rabbit monoclonal (PSH10-87)
reactivity: human
application: western blot

Western blot analysis of Gasdermin D (N terminal) on different lysates with Rabbit anti-Gasdermin D (N terminal) antibody (HA751379) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 35 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751379) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier