Livin
HUABIO
catalog: HA723483
domestic rabbit monoclonal (PSH12-72)
reactivity: human
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of Livin on different lysates with Rabbit anti-Livin antibody (HA723483) at 1/5,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723483) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Livin antibody (HA723483) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723483) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Livin antibody (HA723483) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723483) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
Human Livin
HUABIO
catalog: HA723498
domestic rabbit monoclonal (PSH12-94)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Livin matched pair antibodies Capture: HA723498, Human Livin Rabbit mAb [PSH12-94] Detector: HA723499, Human Livin Rabbit mAb [PSH12-95] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723498) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Angiogenin protein starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723499, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Livin in SK-MEL-28 and HeLa extract samples based on a 1000 µg/ml extract load. Capture: HA723498, Human Livin Rabbit mAb [PSH12-94] Detector: HA723499, Human Livin Rabbit mAb [PSH12-95] Interpolated concentration of native Livin was measured in duplicate at different sample concentrations and interpolated from the Livin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Livin concentration was determined to be 134,381 pg/mL in SK-MEL-28 cell extract. There was no detectable signal in HeLa cell extract.

Interpolated concentrations of spiked Livin in cell culture media samples. Capture: HA723498, Human Livin Rabbit mAb [PSH12-94] Detector: HA723499, Human Livin Rabbit mAb [PSH12-95] The concentrations of Livin were measured in duplicates, interpolated from the Livin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human Livin
HUABIO
catalog: HA723499
domestic rabbit monoclonal (PSH12-95)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Livin matched pair antibodies Capture: HA723498, Human Livin Rabbit mAb [PSH12-94] Detector: HA723499, Human Livin Rabbit mAb [PSH12-95] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723498) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Angiogenin protein starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723499, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human Livin matched pair antibodies Capture: HA723501, Human Livin Rabbit mAb [PSH12-96] Detector: HA723499, Human Livin Rabbit mAb [PSH12-95] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723501) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Angiogenin protein starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723499, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Livin in SK-MEL-28 and HeLa extract samples based on a 1000 µg/ml extract load. Capture: HA723498, Human Livin Rabbit mAb [PSH12-94] Detector: HA723499, Human Livin Rabbit mAb [PSH12-95] Interpolated concentration of native Livin was measured in duplicate at different sample concentrations and interpolated from the Livin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Livin concentration was determined to be 134,381 pg/mL in SK-MEL-28 cell extract. There was no detectable signal in HeLa cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Human Livin
HUABIO
catalog: HA723500B
domestic rabbit monoclonal (PSH12-95)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human Livin matched pair antibodies Capture: HA723498, Human Livin Rabbit mAb [PSH12-94] Detector: HA723499, Human Livin Rabbit mAb [PSH12-95] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723498) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Angiogenin protein starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723499, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human Livin matched pair antibodies Capture: HA723501, Human Livin Rabbit mAb [PSH12-96] Detector: HA723499, Human Livin Rabbit mAb [PSH12-95] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723501) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Angiogenin protein starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723499, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Livin in SK-MEL-28 and HeLa extract samples based on a 1000 µg/ml extract load. Capture: HA723498, Human Livin Rabbit mAb [PSH12-94] Detector: HA723499, Human Livin Rabbit mAb [PSH12-95] Interpolated concentration of native Livin was measured in duplicate at different sample concentrations and interpolated from the Livin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Livin concentration was determined to be 134,381 pg/mL in SK-MEL-28 cell extract. There was no detectable signal in HeLa cell extract.
quantity: 100μl
price: 409.00 USD
to the supplier
Human Livin
HUABIO
catalog: HA723501
domestic rabbit monoclonal (PSH12-96)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Livin matched pair antibodies Capture: HA723501, Human Livin Rabbit mAb [PSH12-96] Detector: HA723499, Human Livin Rabbit mAb [PSH12-95] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723501) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Angiogenin protein starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723499, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Livin in SK-MEL-28 and HeLa extract samples based on a 1000 µg/ml extract load. Capture: HA723501, Human Livin Rabbit mAb [PSH12-96] Detector: HA723499, Human Livin Rabbit mAb [PSH12-95] Interpolated concentration of native Livin was measured in duplicate at different sample concentrations and interpolated from the Livin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Livin concentration was determined to be 53,202 pg/mL in SK-MEL-28 cell extract. There was no detectable signal in HeLa cell extract.

Interpolated concentrations of spiked Livin in cell culture media samples. Capture: HA723501, Human Livin Rabbit mAb [PSH12-96] Detector: HA723499, Human Livin Rabbit mAb [PSH12-95] The concentrations of Livin were measured in duplicates, interpolated from the Livin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Livin
HUABIO
catalog: HA751448
domestic rabbit monoclonal (PSH12-72)
reactivity: human
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of Livin on different lysates with Rabbit anti-Livin antibody (HA751448) at 1/5,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751448) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Livin antibody (HA751448) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751448) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Livin antibody (HA751448) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751448) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier