domestic rabbit monoclonal (SD20-92)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of ATF4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-37, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: PC-12 cell lysate Lane 3: HL-60 cell lysate Lane 4: K562 cell lysate Lane 5: human lung carcinoma tissue lysate
Immunocytochemistry analysis of Neuro-2a cells labeling ATF4 with Rabbit anti-ATF4 antibody (ET1612-37) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ATF4 antibody (ET1612-37) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-ATF4 antibody (ET1612-37) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-37) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 50μl
price: 205.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-08)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
Western blot analysis of ATF-4 on different lysates with Rabbit anti-ATF-4 antibody (HA722796) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: HEK-293 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: C6 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane) Predicted band size: 39 kDa Observed band size: 50 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722796) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ATF-4 on different lysates with Rabbit anti-ATF-4 antibody (HA722796) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: HeLa treated with 250μM CoCl2 for 6 hours cell lysate (20 µg/Lane) Lane 5: NIH/3T3 cell lysate (20 µg/Lane) Lane 6: NIH/3T3 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane) Predicted band size: 39 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722796) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ATF-4 was immunoprecipitated from 0.2 mg HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate with HA722796 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722796 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate (input) Lane 2: HA722796 IP in HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate Lane 3: Rabbit IgG instead of HA722796 in HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 40 seconds; ECL: K1802
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (SD20-92)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of ATF4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750292, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: PC-12 cell lysate Lane 3: HL-60 cell lysate Lane 4: K562 cell lysate Lane 5: human lung carcinoma tissue lysate
Immunocytochemistry analysis of Neuro-2a cells labeling ATF4 with Rabbit anti-ATF4 antibody (HA750292) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ATF4 antibody (HA750292) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-ATF4 antibody (HA750292) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750292) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-08)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
Western blot analysis of ATF-4 on different lysates with Rabbit anti-ATF-4 antibody (HA751130) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: HEK-293 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: C6 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane) Predicted band size: 39 kDa Observed band size: 50 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751130) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ATF-4 on different lysates with Rabbit anti-ATF-4 antibody (HA751130) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: HeLa treated with 250μM CoCl2 for 6 hours cell lysate (20 µg/Lane) Lane 5: NIH/3T3 cell lysate (20 µg/Lane) Lane 6: NIH/3T3 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane) Predicted band size: 39 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751130) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ATF-4 was immunoprecipitated from 0.2 mg HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate with HA751130 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751130 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate (input) Lane 2: HA751130 IP in HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate Lane 3: Rabbit IgG instead of HA751130 in HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 40 seconds; ECL: K1802
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (SD20-92)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section