Mouse IL-33
HUABIO
catalog: HA722985

domestic rabbit monoclonal (PSH08-41)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of mouse IL-33 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722985) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-33 protein (HA210768) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722986, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of mouse IL-33 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722985) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-33 protein (HA210768) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722986, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-33
HUABIO
catalog: HA722986

domestic rabbit monoclonal (PSH08-42)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of mouse IL-33 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722985) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-33 protein (HA210768) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722986, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of mouse IL-33 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722985) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-33 protein (HA210768) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722986, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-33
HUABIO
catalog: HA722987B

domestic rabbit monoclonal (PSH08-42)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of mouse IL-33 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722985) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-33 protein (HA210768) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722987B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of mouse IL-33 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722985) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-33 protein (HA210768) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722987B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier

Mouse IL-33
HUABIO
catalog: HA723863

domestic rabbit monoclonal (PSH16-61)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-33 matched pair antibodies Capture: HA723863, Mouse IL-33 Rabbit mAb [PSH16-61] Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723863) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-33 protein (HA210768) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723864, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-33 in Mouse lung supernatant. Capture: HA723863, Mouse IL-33 Rabbit mAb [PSH16-61] Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62] Interpolated concentration of native IL-33 was measured in duplicate at different sample concentrations and interpolated from the IL-33 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-33 concentration was determined to be 113 pg/ml in Mouse lung supernatant.

Interpolated concentrations of spiked IL-33 in cell culture media samples. Capture: HA723863, Mouse IL-33 Rabbit mAb [PSH16-61] Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62] The concentrations of IL-33 were measured in duplicates, interpolated from the IL-33 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-33
HUABIO
catalog: HA723864

domestic rabbit monoclonal (PSH16-62)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-33 matched pair antibodies Capture: HA723863, Mouse IL-33 Rabbit mAb [PSH16-61] Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723863) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-33 protein (HA210768) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723864, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Mouse IL-33 matched pair antibodies Capture: HA723865, Mouse IL-33 Rabbit mAb [PSH16-63] Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723865) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-33 protein (HA210768) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723864, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-33 in Mouse lung supernatant. Capture: HA723863, Mouse IL-33 Rabbit mAb [PSH16-61] Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62] Interpolated concentration of native IL-33 was measured in duplicate at different sample concentrations and interpolated from the IL-33 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-33 concentration was determined to be 113 pg/ml in Mouse lung supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-33
HUABIO
catalog: HA723864B

domestic rabbit monoclonal (PSH16-62)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse IL-33 matched pair antibodies Capture: HA723863, Mouse IL-33 Rabbit mAb [PSH16-61] Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723863) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-33 protein (HA210768) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723864, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Mouse IL-33 matched pair antibodies Capture: HA723865, Mouse IL-33 Rabbit mAb [PSH16-63] Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723865) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-33 protein (HA210768) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723864, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-33 in Mouse lung supernatant. Capture: HA723863, Mouse IL-33 Rabbit mAb [PSH16-61] Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62] Interpolated concentration of native IL-33 was measured in duplicate at different sample concentrations and interpolated from the IL-33 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-33 concentration was determined to be 113 pg/ml in Mouse lung supernatant.
quantity: 100μl
price: 385.00 USD
to the supplier

Mouse IL-33
HUABIO
catalog: HA723865

domestic rabbit monoclonal (PSH16-63)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-33 matched pair antibodies Capture: HA723865, Mouse IL-33 Rabbit mAb [PSH16-63] Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723865) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-33 protein (HA210768) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723864, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-33 in Mouse lung supernatant . Capture: HA723865, Mouse IL-33 Rabbit mAb [PSH16-63] Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62] Interpolated concentration of native IL-33 was measured in duplicate at different sample concentrations and interpolated from the IL-33 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-33 concentration was determined to be 160 pg/ml in Mouse lung supernatant.

Interpolated concentrations of spiked IL-33 in cell culture media samples. Capture: HA723865, Mouse IL-33 Rabbit mAb [PSH16-63] Detector: HA723864, Mouse IL-33 Rabbit mAb [PSH16-62] The concentrations of IL-33 were measured in duplicates, interpolated from the IL-33 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

IL-33
HUABIO
catalog: HA723920

domestic rabbit monoclonal (PSH17-37)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of IL-33 on different lysates with Rabbit anti-IL-33 antibody (HA723920) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 100nM TPA overnight then 100ng/mL LPS for 7 hours add 1μg/mL BFA for last 3 hours cell lysate Lane 3: Mouse lung tissue lysate Lane 4: Mouse brain tissue lysate (negative) Lane 5: Rat lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 35 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723920) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-IL-33 antibody (HA723920) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723920) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-IL-33 antibody (HA723920) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723920) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

IL-33
HUABIO
catalog: HA751625

domestic rabbit monoclonal (PSH17-37)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of IL-33 on different lysates with Rabbit anti-IL-33 antibody (HA751625) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 100nM TPA overnight then 100ng/mL LPS for 7 hours add 1μg/mL BFA for last 3 hours cell lysate Lane 3: Mouse lung tissue lysate Lane 4: Mouse brain tissue lysate (negative) Lane 5: Rat lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 35 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751625) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-IL-33 antibody (HA751625) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751625) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-IL-33 antibody (HA751625) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751625) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier