Carbonic anhydrase 2
HUABIO
catalog: EM1801-08

mouse monoclonal (11A1)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Carbonic anhydrase 2 on different cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1/1,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell lysate, untreated Lane 2: HL-60 cell lysate, untreated

Western blot analysis of Carbonic anhydrase 2 on different lysates with Mouse anti-Carbonic anhydrase 2 antibody (EM1801-08) at 1/500 dilution. Lane 1: A431 cell lysate Lane 2: 293 cell lysate Lane 3: Human kidney tissue lysate(20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-08) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.

ICC staining Carbonic anhydrase 2 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Carbonic anhydrase 2 monoclonal antibody at a dilution of 1/100 for at least 1 hour at room temperature, washed with PBS. Alexa Fluor™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 360.00 USD
to the supplier

Carbonic anhydrase 2
HUABIO
catalog: EM1801-14

mouse monoclonal (11A2)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Carbonic anhydrase 2 on different lysates with Mouse anti-Carbonic anhydrase 2 antibody (EM1801-14) at 1/1,000 dilution. Lane 1: HL-60 cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of THP-1 cells labeling Carbonic anhydrase 2 with Mouse anti-Carbonic anhydrase 2 antibody (EM1801-14) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Carbonic anhydrase 2 antibody (EM1801-14) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Carbonic anhydrase 2 antibody (EM1801-14) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-14) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

Carbonic anhydrase 2
HUABIO
catalog: ER1802-72

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Carbonic anhydrase 2 on different lysates with Rabbit anti-Carbonic anhydrase 2 antibody (ER1802-72) at 1/500 dilution. Lane 1: 293 cell lysate Lane 2: THP-1 cell lysate Lane 3: HL-60 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-72) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

ICC staining Carbonic anhydrase 2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining Carbonic anhydrase 2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier

Carbonic anhydrase 2
HUABIO
catalog: ET1706-47

domestic rabbit monoclonal (JU12-37)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Striatum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature (recommend).

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature (recommend).

Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-Carbonic anhydrase 2 antibody (ET1706-47) at 1/500 dilution. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature (recommend). The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1706-47, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier

Carbonic anhydrase 2
HUABIO
catalog: HA601241

mouse monoclonal (11A2-R)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Carbonic anhydrase 2 on different lysates with Mouse anti-Carbonic anhydrase 2 antibody (HA601241) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601241) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of THP-1 cells labeling Carbonic anhydrase 2 with Mouse anti-Carbonic anhydrase 2 antibody (HA601241) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Carbonic anhydrase 2 antibody (HA601241) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Carbonic anhydrase 2 antibody (HA601241) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601241) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

Carbonic anhydrase 2
HUABIO
catalog: HA610116

mouse monoclonal (11A2-R)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Carbonic anhydrase 2 on different lysates with Mouse anti-Carbonic anhydrase 2 antibody (HA610116) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610116) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of THP-1 cells labeling Carbonic anhydrase 2 with Mouse anti-Carbonic anhydrase 2 antibody (HA610116) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Carbonic anhydrase 2 antibody (HA610116) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Carbonic anhydrase 2 antibody (HA610116) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610116) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier

Carbonic anhydrase 2
HUABIO
catalog: HA750456

domestic rabbit monoclonal (JU12-37)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Striatum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature (recommend).

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature (recommend).

Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-Carbonic anhydrase 2 antibody (HA750456) at 1/500 dilution. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature (recommend). The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750456, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
quantity: 100μl
price: 649.00 USD
to the supplier