Von Willebrand Factor
HUABIO
catalog: ET1701-87

domestic rabbit monoclonal (JJ09-34)
reactivity: human, mouse
application: western blot

Western blot analysis of Von Willebrand Factor on different lysates with Rabbit anti-Von Willebrand Factor antibody (ET1701-87) at 1/1,000 dilution. Lane 1: bEnd.3 cell lysate Lane 2: Human lung tissue lysate Lane 3: Mouse lung tissue lysate Lane 4: Mouse liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 309 kDa Observed band size: 309 kDa Exposure time: 5 minutes; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-87) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Von Willebrand Factor
HUABIO
catalog: HA722833

domestic rabbit monoclonal (PSH07-45)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of Von Willebrand Factor on different lysates with Rabbit anti-Von Willebrand Factor antibody (HA722833) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution. Lane 1: HEK-293 cell lysate (negative) Lane 2: Human lung tissue lysate Lane 3: Rat lung tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 309 kDa Observed band size: 309 kDa Exposure time: Lane 1-3 (left): 25 seconds; Lane 1-3 (right): 3 minutes; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722833) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-Von Willebrand Factor antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Von Willebrand Factor antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Human Von Willebrand Factor
HUABIO
catalog: HA723058

domestic rabbit monoclonal (PSH09-03)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human Von Willebrand Factor matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723058) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human Von Willebrand Factor protein (HA210992) starting from 60000 pg/ml to 0 pg/ml and detect antibody (HA72359, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Von Willebrand Factor in human samples. Interpolated concentration of native Von Willebrand Factor was measured in duplicate at different sample concentrations and interpolated from the Von Willebrand Factor standard curves. Undiluted samples were 2% human serum and 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Von Willebrand Factor concentration was determined to be 1.73 ug/mL in human serum. There was no detectable signal in Hela cell supernatant.

Interpolated concentrations of spiked Von Willebrand Factor in cell culture media samples. The concentrations of Von Willebrand Factor were measured in duplicates, interpolated from the Von Willebrand Factor standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Human Von Willebrand Factor
HUABIO
catalog: HA723059

domestic rabbit monoclonal (PSH09-04)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human Von Willebrand Factor matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723058) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human Von Willebrand Factor protein (HA210992) starting from 60000 pg/ml to 0 pg/ml and detect antibody (HA72359, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Von Willebrand Factor in human samples. Interpolated concentration of native Von Willebrand Factor was measured in duplicate at different sample concentrations and interpolated from the Von Willebrand Factor standard curves. Undiluted samples were 2% human serum and 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Von Willebrand Factor concentration was determined to be 1.73 ug/mL in human serum. There was no detectable signal in Hela cell supernatant.

Interpolated concentrations of spiked Von Willebrand Factor in cell culture media samples. The concentrations of Von Willebrand Factor were measured in duplicates, interpolated from the Von Willebrand Factor standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Human Von Willebrand Factor
HUABIO
catalog: HA723060B

domestic rabbit monoclonal (PSH09-04)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human Von Willebrand Factor matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723058) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human Von Willebrand Factor protein (HA210992) starting from 60000 pg/ml to 0 pg/ml and detect antibody (HA72360B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Von Willebrand Factor in human samples. Interpolated concentration of native Von Willebrand Factor was measured in duplicate at different sample concentrations and interpolated from the Von Willebrand Factor standard curves. Undiluted samples were 2% human serum and 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Von Willebrand Factor concentration was determined to be 1.73 ug/mL in human serum. There was no detectable signal in Hela cell supernatant.

Interpolated concentrations of spiked Von Willebrand Factor in cell culture media samples. The concentrations of Von Willebrand Factor were measured in duplicates, interpolated from the Von Willebrand Factor standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier

Human Von Willebrand Factor
HUABIO
catalog: HA723431H

domestic rabbit monoclonal (PSH09-04)
reactivity: human
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of human Von Willebrand Factor matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723058) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human Von Willebrand Factor protein (HA210992) starting from 1,000 ng/ml to 0 ng/ml and detect antibody (HA723431H, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier

Von Willebrand Factor
HUABIO
catalog: HA751166

domestic rabbit monoclonal (PSH07-45)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of Von Willebrand Factor on different lysates with Rabbit anti-Von Willebrand Factor antibody (HA751166) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution. Lane 1: HEK-293 cell lysate (negative) Lane 2: Human lung tissue lysate Lane 3: Rat lung tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 309 kDa Observed band size: 309 kDa Exposure time: Lane 1-3 (left): 25 seconds; Lane 1-3 (right): 3 minutes; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751166) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-Von Willebrand Factor antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Von Willebrand Factor antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

Von Willebrand Factor
HUABIO
catalog: IRS215RB

domestic rabbit monoclonal
reactivity: human

mIHC analysis of human liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Von Willebrand Factor antibody (IRS215RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 50T
price: 278.00 USD
to the supplier