domestic rabbit monoclonal (PSH10-32)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
Western blot analysis of HOPX / HOD on different lysates with Rabbit anti-HOPX / HOD antibody (HA723199) at 1/2,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse cerebellum tissue lysate Lane 4: Mouse liver tissue lysate (low expression) Lane 5: Rat lung tissue lysate Lane 6: Rat cerebellum tissue lysate Lane 7: Rat liver tissue lysate (low expression) Lysates/proteins at 20 µg/Lane. Predicted band size: 8 kDa Observed band size: 14 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723199) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-HOPX / HOD antibody (HA723199) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723199) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH10-32)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
Western blot analysis of HOPX / HOD on different lysates with Rabbit anti-HOPX / HOD antibody (HA751349) at 1/2,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse cerebellum tissue lysate Lane 4: Mouse liver tissue lysate (low expression) Lane 5: Rat lung tissue lysate Lane 6: Rat cerebellum tissue lysate Lane 7: Rat liver tissue lysate (low expression) Lysates/proteins at 20 µg/Lane. Predicted band size: 8 kDa Observed band size: 14 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751349) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-HOPX / HOD antibody (HA751349) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751349) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit polyclonal (PSH10-32)
reactivity: mouse, rat
application: western blot, immunohistochemistry - frozen section
reactivity: mouse, rat
application: western blot, immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required
Western blot analysis of HOPX / HOD on different lysates with Rat anti-HOPX / HOD antibody (HA601484) at 1/2,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse liver tissue lysate (low expression) Lane 4: Rat lung tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 8 kDa Observed band size: 14 kDa Exposure time: 2 minutes 41 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601484) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier
domestic rabbit polyclonal (PSH10-32)
reactivity: mouse, rat
application: western blot, immunohistochemistry - frozen section
reactivity: mouse, rat
application: western blot, immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required
Western blot analysis of HOPX / HOD on different lysates with Rat anti-HOPX / HOD antibody (HA610318) at 1/2,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse liver tissue lysate (low expression) Lane 4: Rat lung tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 8 kDa Observed band size: 14 kDa Exposure time: 2 minutes 41 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610318) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μg
price: 649.00 USD
to the supplier