domestic rabbit monoclonal (PSH10-75)
reactivity: human, mouse
application: ELISA
reactivity: human, mouse
application: ELISA
Sandwich ELISA analysis of Human VEGFC matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723241) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGFC protein (HA210869) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723242, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native VEGF-C in human serum and mouse serum samples. The concentrations of VEGF-C were measured in duplicates, interpolated from the VEGF-C standard curve and corrected for sample dilution. Undiluted samples are human serum 100% and mouse serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF-C concentration was determined to be 236.5 pg/ml in human serum and 115.4 pg/ml in mouse serum.
Interpolated concentrations of spiked VEGF-C in human cell culture media samples. The concentrations of VEGF-C were measured in duplicate, interpolated from the VEGF-C standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%, The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH10-76)
reactivity: human, mouse
application: ELISA
reactivity: human, mouse
application: ELISA
Sandwich ELISA analysis of Human VEGFC matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723241) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGFC protein (HA210869) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723242, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native VEGF-C in human serum and mouse serum samples. The concentrations of VEGF-C were measured in duplicates, interpolated from the VEGF-C standard curve and corrected for sample dilution. Undiluted samples are human serum 100% and mouse serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF-C concentration was determined to be 236.5 pg/ml in human serum and 115.4 pg/ml in mouse serum.
Interpolated concentrations of spiked VEGF-C in human cell culture media samples. The concentrations of VEGF-C were measured in duplicate, interpolated from the VEGF-C standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%, The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH10-76)
reactivity: human, mouse
conjugate: biotin
application: ELISA
reactivity: human, mouse
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Human VEGFC matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723241) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGFC protein (HA210869) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723243B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native VEGF-C in human serum and mouse serum samples. The concentrations of VEGF-C were measured in duplicates, interpolated from the VEGF-C standard curve and corrected for sample dilution. Undiluted samples are human serum 100% and mouse serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF-C concentration was determined to be 236.5 pg/ml in human serum and 115.4 pg/ml in mouse serum.
Interpolated concentrations of spiked VEGF-C in human cell culture media samples. The concentrations of VEGF-C were measured in duplicate, interpolated from the VEGF-C standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%, The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Western blot analysis of VEGFC on different lysates with Rabbit anti-VEGFC antibody (R1307-4) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: 293 cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 47/100 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-4) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier