VEGF
HUABIO
catalog: ER30607
domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of VEGF on different lysates with Rabbit anti-VEGF antibody (ER30607) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: HUVEC cell lysate Lane 4: MCF7 cell lysate Lane 5: U-87 MG cell lysate Lane 6: K-562 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER30607) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

All lanes: Western blot analysis of VEGF with anti-VEGF antibody (ER30607) at 1:500 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2/3: VEGF knockdown Hela whole cell lysate (10 µg). ER30607 was shown to specifically react with VEGF in wild-type Hela cells. Weakened bands were observed when VEGF knockdown samples were tested. Wild-type and VEGF knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER30607, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

ICC staining VEGF in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier
VEGF
HUABIO
catalog: ET1604-28-50UL
domestic rabbit monoclonal (SP07-01)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Immunocytochemistry analysis of HUVEC cells labeling VEGF with Rabbit anti-VEGF antibody (ET1604-28) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VEGF antibody (ET1604-28) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of NIH/3T3 cells labeling VEGF with Rabbit anti-VEGF antibody (ET1604-28) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VEGF antibody (ET1604-28) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-VEGF antibody (ET1604-28) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-28) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 50μl
price: 205.00 USD
to the supplier
Human VEGFA
HUABIO
catalog: HA723493
domestic rabbit monoclonal (PSH12-91)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human VEGFA matched pair antibodies Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91] Detector: HA723494, Human VEGFA Rabbit mAb [PSH12-92] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723493) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGFA protein (HA210929) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723494, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human VEGFA matched pair antibodies Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91] Detector: HA723496, Human VEGFA Rabbit mAb [PSH12-93] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723493) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGFA protein (HA210929) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723496, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native VEGFA in A431 and HepG2 cell culture supernatant. Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91] Detector: HA723494, Human VEGFA Rabbit mAb [PSH12-92] The concentrations of VEGFA measured in duplicate and interpolated from the VEGFA standard curve and corrected for sample dilution. Undiluted samples are as follows: A431 cell culture supernatant 12.5% and HepG2 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGFA concentration was determined to be 6,033 pg/ml in A431 cell culture supernatant and 3,949 pg/ml in HepG2 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
Human VEGFA
HUABIO
catalog: HA723494
domestic rabbit monoclonal (PSH12-92)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human VEGFA matched pair antibodies Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91] Detector: HA723494, Human VEGFA Rabbit mAb [PSH12-92] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723493) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGFA protein (HA210929) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723494, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native VEGFA in A431 and HepG2 cell culture supernatant. Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91] Detector: HA723494, Human VEGFA Rabbit mAb [PSH12-92] The concentrations of VEGFA measured in duplicate and interpolated from the VEGFA standard curve and corrected for sample dilution. Undiluted samples are as follows: A431 cell culture supernatant 12.5% and HepG2 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGFA concentration was determined to be 6,033 pg/ml in A431 cell culture supernatant and 3,949 pg/ml in HepG2 cell culture supernatant.

Interpolated concentrations of spiked VEGFA in human cell culture media samples. Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91] Detector: HA723494, Human VEGFA Rabbit mAb [PSH12-92] The concentrations of VEGFA were measured in duplicates, interpolated from the VEGFAstandard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human VEGFA
HUABIO
catalog: HA723495B
domestic rabbit monoclonal (PSH12-92)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human VEGFA matched pair antibodies Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91] Detector: HA723494, Human VEGFA Rabbit mAb [PSH12-92] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723493) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGFA protein (HA210929) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723494, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native VEGFA in A431 and HepG2 cell culture supernatant. Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91] Detector: HA723494, Human VEGFA Rabbit mAb [PSH12-92] The concentrations of VEGFA measured in duplicate and interpolated from the VEGFA standard curve and corrected for sample dilution. Undiluted samples are as follows: A431 cell culture supernatant 12.5% and HepG2 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGFA concentration was determined to be 6,033 pg/ml in A431 cell culture supernatant and 3,949 pg/ml in HepG2 cell culture supernatant.

Interpolated concentrations of spiked VEGFA in human cell culture media samples. Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91] Detector: HA723494, Human VEGFA Rabbit mAb [PSH12-92] The concentrations of VEGFA were measured in duplicates, interpolated from the VEGFAstandard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
Human VEGFA
HUABIO
catalog: HA723496
domestic rabbit monoclonal (PSH12-93)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human VEGFA matched pair antibodies Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91] Detector: HA723496, Human VEGFA Rabbit mAb [PSH12-93] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723493) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGFA protein (HA210929) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723496, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native VEGFA in A431 and HepG2 cell culture supernatant. Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91] Detector: HA723496, Human VEGFA Rabbit mAb [PSH12-93] The concentrations of VEGFA measured in duplicate and interpolated from the VEGFA standard curve and corrected for sample dilution. Undiluted samples are as follows: A431 cell culture supernatant 12.5% and HepG2 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGFA concentration was determined to be 6,139 pg/ml in A431 cell culture supernatant and 3,773 pg/ml in HepG2 cell culture supernatant.

Interpolated concentrations of spiked VEGF in human cell culture media samples. Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91] Detector: HA723496, Human VEGFA Rabbit mAb [PSH12-93] The concentrations of VEGFA were measured in duplicates, interpolated from the VEGFA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human VEGFA
HUABIO
catalog: HA723497B
domestic rabbit monoclonal (PSH12-93)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human VEGFA matched pair antibodies Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91] Detector: HA723496, Human VEGFA Rabbit mAb [PSH12-93] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723493) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGFA protein (HA210929) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723496, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native VEGFA in A431 and HepG2 cell culture supernatant. Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91] Detector: HA723496, Human VEGFA Rabbit mAb [PSH12-93] The concentrations of VEGFA measured in duplicate and interpolated from the VEGFA standard curve and corrected for sample dilution. Undiluted samples are as follows: A431 cell culture supernatant 12.5% and HepG2 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGFA concentration was determined to be 6,139 pg/ml in A431 cell culture supernatant and 3,773 pg/ml in HepG2 cell culture supernatant.

Interpolated concentrations of spiked VEGF in human cell culture media samples. Capture: HA723493, Human VEGFA Rabbit mAb [PSH12-91] Detector: HA723496, Human VEGFA Rabbit mAb [PSH12-93] The concentrations of VEGFA were measured in duplicates, interpolated from the VEGFA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
VEGF
HUABIO
catalog: HA750074
domestic rabbit monoclonal (SP07-01)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Immunocytochemistry analysis of HUVEC cells labeling VEGF with Rabbit anti-VEGF antibody (HA750074) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VEGF antibody (HA750074) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of NIH/3T3 cells labeling VEGF with Rabbit anti-VEGF antibody (HA750074) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VEGF antibody (HA750074) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-VEGF antibody (HA750074) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750074) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier