VCAM1
HUABIO
catalog: ET1601-18-50UL
domestic rabbit monoclonal (SA05-04)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of VCAM1 on different lysates with Rabbit anti-VCAM1 antibody (ET1601-18) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: C2C12 cell lysate (20 µg/Lane) Lane 2: 3T3-L1 cell lysate (20 µg/Lane) Lane 3: Mouse spleen tissue lysate (20 µg/Lane) Lane 4: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 81 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-18) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of VCAM1 on different lysates with Rabbit anti-VCAM1 antibody (ET1601-18) at 1/5,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: C2C12 cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (negative) (20 µg/Lane) Lane 4: Mouse spleen tissue lysate (20 µg/Lane) Lane 5: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 81 kDa Observed band size: 100 kDa Exposure time: 24 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-18) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-VCAM1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-18, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 50μl
price: 205.00 USD
to the supplier
Human VCAM-1
HUABIO
catalog: HA725064
domestic rabbit monoclonal (PSH12-46)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human VCAM-1 matched pair antibodies Capture: HA725064, Human VCAM-1 Rabbit mAb [PSH12-46] Detector: HA725065, Human VCAM-1 Rabbit mAb [PSH12-47] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725064) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VCAM-1 protein (HA210976) protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725065, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native VCAM-1 in human samples. Capture: HA725064, Human VCAM-1 Rabbit mAb [PSH12-46] Detector: HA725065, Human VCAM-1 Rabbit mAb [PSH12-47] Interpolated concentration of native VCAM-1 was measured in duplicate at different sample concentrations. Undiluted samples were 1% human serum. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean VCAM-1 concentration was determined to be 1,361,999 pg/mL in human serum. There was no detectable signal in SK-OV-3 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
Human VCAM-1
HUABIO
catalog: HA725065
domestic rabbit monoclonal (PSH12-47)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human VCAM-1 matched pair antibodies Capture: HA725064, Human VCAM-1 Rabbit mAb [PSH12-46] Detector: HA725065, Human VCAM-1 Rabbit mAb [PSH12-47] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725064) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VCAM-1 protein (HA210976) protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725065, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native VCAM-1 in human samples. Capture: HA725064, Human VCAM-1 Rabbit mAb [PSH12-46] Detector: HA725065, Human VCAM-1 Rabbit mAb [PSH12-47] Interpolated concentration of native VCAM-1 was measured in duplicate at different sample concentrations. Undiluted samples were 1% human serum. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean VCAM-1 concentration was determined to be 1,361,999 pg/mL in human serum. There was no detectable signal in SK-OV-3 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
Human VCAM-1
HUABIO
catalog: HA725066B
domestic rabbit monoclonal (PSH12-47)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human VCAM-1 matched pair antibodies Capture: HA725064, Human VCAM-1 Rabbit mAb [PSH12-46] Detector: HA725065, Human VCAM-1 Rabbit mAb [PSH12-47] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725064) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VCAM-1 protein (HA210976) protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725065, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native VCAM-1 in human samples. Capture: HA725064, Human VCAM-1 Rabbit mAb [PSH12-46] Detector: HA725065, Human VCAM-1 Rabbit mAb [PSH12-47] Interpolated concentration of native VCAM-1 was measured in duplicate at different sample concentrations. Undiluted samples were 1% human serum. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean VCAM-1 concentration was determined to be 1,361,999 pg/mL in human serum. There was no detectable signal in SK-OV-3 cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier
Human VCAM-1
HUABIO
catalog: HA725066H
domestic rabbit monoclonal (PSH12-47)
reactivity: human
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of Human VCAM-1 matched pair antibodies Capture: HA725064, Human VCAM-1 Rabbit mAb [PSH12-46] Detector: HA725065, Human VCAM-1 Rabbit mAb [PSH12-47] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725064) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VCAM-1 protein (HA210976) protein starting from 333,000 pg/ml to 0 pg/ml and detect antibody (HA725065, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier
VCAM1
HUABIO
catalog: HA750009
domestic rabbit monoclonal (SA05-04)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of VCAM1 on different lysates with Rabbit anti-VCAM1 antibody (HA750009) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: C2C12 cell lysate (20 µg/Lane) Lane 2: 3T3-L1 cell lysate (20 µg/Lane) Lane 3: Mouse spleen tissue lysate (20 µg/Lane) Lane 4: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 81 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750009) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of VCAM1 on different lysates with Rabbit anti-VCAM1 antibody (HA750009) at 1/5,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: C2C12 cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (negative) (20 µg/Lane) Lane 4: Mouse spleen tissue lysate (20 µg/Lane) Lane 5: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 81 kDa Observed band size: 100 kDa Exposure time: 24 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750009) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-VCAM1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750009, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
VCAM1
HUABIO
catalog: R1512-13
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of VCAM1 on human spleen tissue lysate using anti-VCAM1 antibody at 1/5,000 dilution.

ICC staining VCAM1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining VCAM1 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier