CD134
HUABIO
catalog: EM1701-24
mouse monoclonal (2H1)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD134 on mouse lung tissue lysates with Mouse anti-CD134 antibody (EM1701-24) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-24) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

ICC staining CD134 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining CD134 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 360.00 USD
to the supplier
CD134
HUABIO
catalog: HA722700
domestic rabbit monoclonal (JE36-48)
reactivity: human
application: western blot

Western blot analysis of CD134 on different lysates with Rabbit anti-CD134 antibody (HA722700) at 1/1,000 dilution. Lane 1: HUT 102 cell lysate Lane 2: Jurkat cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 50 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
CD134
HUABIO
catalog: HA723183
domestic rabbit monoclonal (PSH10-14)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry

Western blot analysis of CD134 on different lysates with Rabbit anti-CD134 antibody (HA723183) at 1/2,000 dilution. Lane 1: HUT 102 cell lysate Lane 2: Jurkat cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 50 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723183) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HUT 102 (positive) and Jurkat (negative) labeling CD134 with Rabbit anti-CD134 antibody (HA723183) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD134 antibody (HA723183) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of Jurkat (left, negative) and HUT 102 (right, positive) cells labeling CD134. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723183, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
Human OX40/TNFRSF4
HUABIO
catalog: HA723187
domestic rabbit monoclonal (PSH10-17)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human OX40/TNFRSF4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723187) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human OX40/TNFRSF4 protein (HA210892) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723188, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native OX40 in human PBMC cell culture supernatant. PBMC cells were stimulated with 10 µg/ml PHA-M or vehicle control and incubated for 5 days. The concentrations of OX40 measured, interpolated from the OX40 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The mean OX40 concentration was determined to be 418.6 pg/ml in PHA-M stimulated PBMC cell culture supernatant and undetectable in the unstimulated PBMC control.

Interpolated concentrations of spiked OX40 in human cell culture media samples. The concentrations of OX40 were interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 1 50%, cell culture media 2 50%.
quantity: 100μl
price: 649.00 USD
to the supplier
Human OX40/TNFRSF4
HUABIO
catalog: HA723188
domestic rabbit monoclonal (PSH10-18)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human OX40/TNFRSF4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723187) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human OX40/TNFRSF4 protein (HA210892) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723188, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native OX40 in human PBMC cell culture supernatant. PBMC cells were stimulated with 10 µg/ml PHA-M or vehicle control and incubated for 5 days. The concentrations of OX40 measured, interpolated from the OX40 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The mean OX40 concentration was determined to be 418.6 pg/ml in PHA-M stimulated PBMC cell culture supernatant and undetectable in the unstimulated PBMC control.

Interpolated concentrations of spiked OX40 in human cell culture media samples. The concentrations of OX40 were interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 1 50%, cell culture media 2 50%.
quantity: 100μl
price: 649.00 USD
to the supplier
Human OX40/TNFRSF4
HUABIO
catalog: HA723189B
domestic rabbit monoclonal (PSH10-18)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human OX40/TNFRSF4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723187) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human OX40/TNFRSF4 protein (HA210892) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723189B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native OX40 in human PBMC cell culture supernatant. PBMC cells were stimulated with 10 µg/ml PHA-M or vehicle control and incubated for 5 days. The concentrations of OX40 measured, interpolated from the OX40 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The mean OX40 concentration was determined to be 418.6 pg/ml in PHA-M stimulated PBMC cell culture supernatant and undetectable in the unstimulated PBMC control.

Interpolated concentrations of spiked OX40 in human cell culture media samples. The concentrations of OX40 were interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 1 50%, cell culture media 2 50%.
quantity: 100μl
price: 409.00 USD
to the supplier
CD134
HUABIO
catalog: HA751343
domestic rabbit monoclonal (PSH10-14)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry

Western blot analysis of CD134 on different lysates with Rabbit anti-CD134 antibody (HA751343) at 1/2,000 dilution. Lane 1: HUT 102 cell lysate Lane 2: Jurkat cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 50 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751343) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HUT 102 (positive) and Jurkat (negative) labeling CD134 with Rabbit anti-CD134 antibody (HA751343) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD134 antibody (HA751343) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of Jurkat (left, negative) and HUT 102 (right, positive) cells labeling CD134. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751343, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier