HUABIO alpha-tubulin antibody

alpha Tubulin
HUABIO
catalog: ER130905

domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (ER130905) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: PC-12 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: Daudi cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: A431 cell lysate (20 µg/Lane) Lane 7: K-562 cell lysate (20 µg/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER130905) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (ER130905) at different dilutions. Lane 1/2: NIH3T3 cell lysate at 1/1,000 and 1/5,000 dilution Lane 3/4: HepG2 cell lysate at 1/1,000 and 1/5,000 dilution Lane 5/6: PC-12 cell lysate at 1/1,000 and 1/5,000 dilution Lane 7/8: Hela cell lysate at 1/1,000 and 1/5,000 dilution Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER130905) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

ICC staining of alpha Tubulin in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER130905, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 330 USD
to the supplier

alpha Tubulin 4A
HUABIO
catalog: ET1705-31

domestic rabbit monoclonal (JM73-24)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of alpha Tubulin 4A on different lysates with Rabbit anti-alpha Tubulin 4A antibody (ET1705-31) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: PC-12 cell lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: Lane 1-2: 8 seconds; Lane 3-4: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-31) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of alpha Tubulin 4A on different lysates with Rabbit anti-alpha Tubulin 4A antibody (ET1705-31) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-alpha Tubulin 4A KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-31) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells labeling alpha Tubulin 4A with Rabbit anti-alpha Tubulin 4A antibody (ET1705-31) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin 4A antibody (ET1705-31) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier

alpha Tubulin
HUABIO
catalog: HA500366

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500366) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of A431 cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Immunocytochemistry analysis of SH-SY5Y cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 330 USD
to the supplier

L-Lactyl-alpha Tubulin (K40)
HUABIO
catalog: HA500554

domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section

Dotblot: 1.Lac-tub primary antibody, 1:10,000 dilution (HA500554). 2. Exposure time: 60 s 3. 0.45 μm NC membrane

WB: 1.Lac-tub primary antibody, 1:2,000 dilution (HA500554). 2. HEK293T cell lysate from cells that were transfected with HA-tagged α-tubulinWT or α-tubulinK40A plasmids. Predicted band size: 50 kDa, Observed band size: 50 kDa. Exposure time: 30 s;10% SDS-PAGE gel. Proteins were transferred to a 0.45 μm NC membrane and blocked with 5% milk without fat in TBST for 0.5 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% BSA in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:10,000 dilution was used for 1 hour at room temperature.

WB: 1.Lac-tub primary antibody, 1:2,000 dilution (HA500554). 2. HEK293T cell lysate from cells that were treated with sodium lactate. Predicted band size: 50 kDa, Observed band size: 50 kDa. Exposure time: 30 s;10% SDS-PAGE gel.Proteins were transferred to a NC membrane and blocked with 5% milk without fat in TBST for 0.5 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% BSA in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:10,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier

alpha Tubulin
HUABIO
catalog: HA721272

domestic rabbit monoclonal (PSH0-14)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (HA721272) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: A431 cell lysate Lane 3: HepG2 cell lysate Lane 4: 293 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721272) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (HA721272) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: SH-SY5Y cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721272) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (HA721272) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721272) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

alpha Tubulin
HUABIO
catalog: HA721913

domestic rabbit monoclonal (PSH02-94)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (HA721913) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721913) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (HA721913) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (HA721913) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of K-562 cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (HA721913) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (HA721913) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier

alpha Tubulin
HUABIO
catalog: HA721914

domestic rabbit monoclonal (PSH02-95)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (HA721914) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721914, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-alpha Tubulin antibody (HA721914) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721914) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

alpha Tubulin
HUABIO
catalog: HA750584

domestic rabbit monoclonal (PSH0-14)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (HA750584) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: A431 cell lysate Lane 3: HepG2 cell lysate Lane 4: 293 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750584) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (HA750584) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: SH-SY5Y cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750584) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (HA750584) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750584) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

alpha Tubulin
HUABIO
catalog: HA750849

domestic rabbit monoclonal (PSH02-94)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (HA750849) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750849) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (HA750849) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (HA750849) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of K-562 cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (HA750849) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (HA750849) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier

alpha Tubulin
HUABIO
catalog: HA750850

domestic rabbit monoclonal (PSH02-95)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section