domestic rabbit monoclonal (PSH13-88)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
Western blot analysis of TRAF1 on different lysates with Rabbit anti-TRAF1 antibody (HA723581) at 1/10,000 dilution. Lane 1: Raji cell lysate (20 µg/Lane) Lane 2: HEK-293 cell lysate (negative) (20 µg/Lane) Lane 3: Ramos cell lysate (20 µg/Lane) Lane 4: Daudi cell lysate (20 µg/Lane) Lane 5: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723581) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
TRAF1 was immunoprecipitated from 0.2 mg Raji cell lysate with HA723581 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723581 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Raji cell lysate (input) Lane 2: HA723581 IP in Raji cell lysate Lane 3: Rabbit IgG instead of HA723581 in Raji cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 10 seconds; ECL: K1801
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-27)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human TRAF1 matched pair antibodies Capture: HA723618, Human TRAF1 Rabbit mAb [PSH14-27] Detector: HA723619, Human TRAF1 Rabbit mAb [PSH14-28] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723618) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TRAF1 protein (HA211289) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723619, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native TRAF1 in Raji and HEK-293 extract samples based on a 1,000 µg/ml extract load. Capture: HA723618, Human TRAF1 Rabbit mAb [PSH14-27] Detector: HA723619, Human TRAF1 Rabbit mAb [PSH14-28] The concentrations of TRAF1 were measured in duplicates, interpolated from the TRAF1 standard curve and corrected for sample dilution. Undiluted samples are Raji extract 20% and HEK-293 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TRAF1 concentration was determined to be 10,599 pg/ml in Raji extract and undetectable in HEK-293 extract.
Interpolated concentrations of spiked TRAF1 in human cell culture media samples. Capture: HA723618, Human TRAF1 Rabbit mAb [PSH14-27] Detector: HA723619, Human TRAF1 Rabbit mAb [PSH14-28] The concentrations of TRAF1 were measured in duplicates, interpolated from the TRAF1standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-28)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human TRAF1 matched pair antibodies Capture: HA723618, Human TRAF1 Rabbit mAb [PSH14-27] Detector: HA723619, Human TRAF1 Rabbit mAb [PSH14-28] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723618) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TRAF1 protein (HA211289) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723619, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native TRAF1 in Raji and HEK-293 extract samples based on a 1,000 µg/ml extract load. Capture: HA723618, Human TRAF1 Rabbit mAb [PSH14-27] Detector: HA723619, Human TRAF1 Rabbit mAb [PSH14-28] The concentrations of TRAF1 were measured in duplicates, interpolated from the TRAF1 standard curve and corrected for sample dilution. Undiluted samples are Raji extract 20% and HEK-293 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TRAF1 concentration was determined to be 10,599 pg/ml in Raji extract and undetectable in HEK-293 extract.
Interpolated concentrations of spiked TRAF1 in human cell culture media samples. Capture: HA723618, Human TRAF1 Rabbit mAb [PSH14-27] Detector: HA723619, Human TRAF1 Rabbit mAb [PSH14-28] The concentrations of TRAF1 were measured in duplicates, interpolated from the TRAF1standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-28)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Human TRAF1 matched pair antibodies Capture: HA723618, Human TRAF1 Rabbit mAb [PSH14-27] Detector: HA723619, Human TRAF1 Rabbit mAb [PSH14-28] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723618) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TRAF1 protein (HA211289) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723619, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native TRAF1 in Raji and HEK-293 extract samples based on a 1,000 µg/ml extract load. Capture: HA723618, Human TRAF1 Rabbit mAb [PSH14-27] Detector: HA723619, Human TRAF1 Rabbit mAb [PSH14-28] The concentrations of TRAF1 were measured in duplicates, interpolated from the TRAF1 standard curve and corrected for sample dilution. Undiluted samples are Raji extract 20% and HEK-293 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TRAF1 concentration was determined to be 10,599 pg/ml in Raji extract and undetectable in HEK-293 extract.
Interpolated concentrations of spiked TRAF1 in human cell culture media samples. Capture: HA723618, Human TRAF1 Rabbit mAb [PSH14-27] Detector: HA723619, Human TRAF1 Rabbit mAb [PSH14-28] The concentrations of TRAF1 were measured in duplicates, interpolated from the TRAF1standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit monoclonal (PSH13-88)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
Western blot analysis of TRAF1 on different lysates with Rabbit anti-TRAF1 antibody (HA751490) at 1/10,000 dilution. Lane 1: Raji cell lysate (20 µg/Lane) Lane 2: HEK-293 cell lysate (negative) (20 µg/Lane) Lane 3: Ramos cell lysate (20 µg/Lane) Lane 4: Daudi cell lysate (20 µg/Lane) Lane 5: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751490) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
TRAF1 was immunoprecipitated from 0.2 mg Raji cell lysate with HA751490 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751490 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Raji cell lysate (input) Lane 2: HA751490 IP in Raji cell lysate Lane 3: Rabbit IgG instead of HA751490 in Raji cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 10 seconds; ECL: K1801
quantity: 100μl
price: 649.00 USD
to the supplier