Complement C3 (beta chain)
HUABIO
catalog: 0809-8

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis on human serum using anti-complement C3 polyclonal antibody. Note: 50kD band is caused by nonspecific reaction (goat anti-rabbit to human heavy chain).
quantity: 100μl
price: 330 USD
to the supplier

Complement C3
HUABIO
catalog: ET1702-99

domestic rabbit monoclonal (JF10-30)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of Complement C3 on different lysates with Rabbit anti-Complement C3 antibody (ET1702-99) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse spleen tissue lysate Lane 6: Rat liver tissue lysate Lane 7: Rat spleen tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 187 kDa Observed band size: 108/37 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-99) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HepG2 cells labeling Complement C3 with Rabbit anti-Complement C3 antibody (ET1702-99) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Complement C3 antibody (ET1702-99) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of HepG2 cells labeling Complement C3. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-99, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier

Complement C3
HUABIO
catalog: ET7106-75

domestic rabbit monoclonal (JU42-68)
reactivity: human
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of C3 on SiHa cell lysates using anti-C3 antibody at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-C3 antibody. Counter stained with hematoxylin.

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-C3 antibody. Counter stained with hematoxylin.
quantity: 100μl
price: 385.00 USD
to the supplier

Human Complement C3
HUABIO
catalog: HA725140

domestic rabbit monoclonal (PSH15-07)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Complement C3 matched pair antibodies Capture: HA725140, Human Complement C3 Rabbit mAb [PSH15-07] Detector: HA725141, Human Complement C3 Rabbit mAb [PSH15-08] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725140) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Complement C3 protein (HA211092) starting from 400,000 pg/ml to 0 pg/ml and detect antibody (HA725141, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Complement C3 in HepG2 cell culture supernatant. Capture: HA725140, Human Complement C3 Rabbit mAb [PSH15-07] Detector: HA725141, Human Complement C3 Rabbit mAb [PSH15-08] The concentrations of Complement C3 were measured in duplicates, interpolated from the Complement C3 standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C3 concentration was determined to be 6,576 ng/ml in HepG2 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Complement C3
HUABIO
catalog: HA725141

domestic rabbit monoclonal (PSH15-08)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Complement C3 matched pair antibodies Capture: HA725140, Human Complement C3 Rabbit mAb [PSH15-07] Detector: HA725141, Human Complement C3 Rabbit mAb [PSH15-08] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725140) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Complement C3 protein (HA211092) starting from 400,000 pg/ml to 0 pg/ml and detect antibody (HA725141, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Complement C3 in HepG2 cell culture supernatant. Capture: HA725140, Human Complement C3 Rabbit mAb [PSH15-07] Detector: HA725141, Human Complement C3 Rabbit mAb [PSH15-08] The concentrations of Complement C3 were measured in duplicates, interpolated from the Complement C3 standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C3 concentration was determined to be 6,576 ng/ml in HepG2 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Complement C3
HUABIO
catalog: HA725142B

domestic rabbit monoclonal (PSH15-08)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Complement C3 matched pair antibodies Capture: HA725140, Human Complement C3 Rabbit mAb [PSH15-07] Detector: HA725141, Human Complement C3 Rabbit mAb [PSH15-08] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725140) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Complement C3 protein (HA211092) starting from 400,000 pg/ml to 0 pg/ml and detect antibody (HA725141, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Complement C3 in HepG2 cell culture supernatant. Capture: HA725140, Human Complement C3 Rabbit mAb [PSH15-07] Detector: HA725141, Human Complement C3 Rabbit mAb [PSH15-08] The concentrations of Complement C3 were measured in duplicates, interpolated from the Complement C3 standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C3 concentration was determined to be 6,576 ng/ml in HepG2 cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier

Human Complement C3
HUABIO
catalog: HA725142H

domestic rabbit monoclonal (PSH15-08)
reactivity: human
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of Complement C3 matched pair antibodies Capture: HA725140, Human Complement C3 Rabbit mAb [PSH15-07] Detector: HA725141, Human Complement C3 Rabbit mAb [PSH15-08] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725140) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Complement C3 protein (HA211092) starting from 1,000,000 pg/ml to 0 pg/ml and detect antibody (HA725141, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier

Complement C3
HUABIO
catalog: HA750367

domestic rabbit monoclonal (JF10-30)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of Complement C3 on different lysates with Rabbit anti-Complement C3 antibody (HA750367) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse spleen tissue lysate Lane 6: Rat liver tissue lysate Lane 7: Rat spleen tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 187 kDa Observed band size: 108/37 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750367) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HepG2 cells labeling Complement C3 with Rabbit anti-Complement C3 antibody (HA750367) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Complement C3 antibody (HA750367) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of HepG2 cells labeling Complement C3. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750367, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier

Complement C3 (beta chain)
HUABIO
catalog: M0809-14

mouse monoclonal (11-10)
reactivity: human
application: western blot, flow cytometry

Western blot analysis on human plasma lysates using anti-C3(β-chain) Mouse mAb (Cat. # M0809-14).
quantity: 100μl
price: 360.00 USD
to the supplier

Complement C3
HUABIO
catalog: M1510-39

mouse monoclonal (6-A10)
reactivity: human
application: western blot, ELISA, immunohistochemistry - paraffin section

1. High specificity Due to their recognition of only one epitope, mAbs are best known for their high specificity. Producing highly specific monoclonal antibodies supplies researchers with the benefits of improved target identification and less background signal. 2. Batch-to-batch reproducibility Since a monoclonal antibody is made by cloning a unique while blood cell. All subsequent products developed from this antibody trace back to its original, unique parent cell. This makes for great batch-to-batch reproducibility. 3. Guaranteed long term supply Hybridoma serves as a continuous source of monoclonal antibody.
quantity: 100μl
price: 360.00 USD
to the supplier

Complement C3
HUABIO
catalog: M1510-40

mouse monoclonal (10-B3)
reactivity: human
application: western blot, ELISA, immunohistochemistry - paraffin section

1. High specificity Due to their recognition of only one epitope, mAbs are best known for their high specificity. Producing highly specific monoclonal antibodies supplies researchers with the benefits of improved target identification and less background signal. 2. Batch-to-batch reproducibility Since a monoclonal antibody is made by cloning a unique while blood cell. All subsequent products developed from this antibody trace back to its original, unique parent cell. This makes for great batch-to-batch reproducibility. 3. Guaranteed long term supply Hybridoma serves as a continuous source of monoclonal antibody.
quantity: 100μl
price: 360.00 USD
to the supplier