TAK1
HUABIO
catalog: ER1802-90

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: flow cytometry, immunohistochemistry - paraffin section

ICC staining TAK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining TAK1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining TAK1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier

TAK1
HUABIO
catalog: ET1705-14

domestic rabbit monoclonal (JM73-19)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of TAK1 on different lysates with Rabbit anti-TAK1 antibody (ET1705-14) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD TAK1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 67 kDa Observed band size: 72 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-14) at 1/1,000 dilution was used in 2% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of TAK1 on different lysates with Rabbit anti-TAK1 antibody (ET1705-14) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Mouse lung tissue lysate (40 µg/Lane) Lane 6: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 67 kDa Observed band size: 72 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of TAK1 on different lysates with Rabbit anti-TAK1 antibody (ET1705-14) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-TAK1 (S439)
HUABIO
catalog: HA722242

domestic rabbit monoclonal (JE44-93)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-TAK1 (S439) on different lysates with Rabbit anti-Phospho-TAK1 (S439) antibody (HA722242) at 1/25,000 dilution. Lane 1: K-562 cell lysate Lane 2: C2C12 cell lysate Lane 3: C6 cell lysate Lane 4: HeLa cell lysate Lane 5: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: NIH/3T3 starved overnight then treated with 100nM Calyculin A for 30 minutes cell lysate Lane 8: PC-12 cell lysate Lane 9: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722242) at 1/25,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Phospho-TAK1 (S439) on different lysates with Rabbit anti-Phospho-TAK1 (S439) antibody (HA722242) at 1/5,000 dilution and pan TAK1 antibody (ET1705-14) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lane 3: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722242) at 1/5,000 dilution and pan TAK1 antibody (ET1705-14) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-TAK1 (S412)
HUABIO
catalog: HA723662

domestic rabbit monoclonal (PSH14-64)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of Phospho-TAK1 (S412) on different lysates with Rabbit anti-Phospho-TAK1 (S412) antibody (HA723662) at 1/5,000 dilution. Lane 1: K-562 cell lysate Lane 2: C2C12 cell lysate Lane 3: C6 cell lysate Lane 4: HeLa cell lysate Lane 5: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: NIH/3T3 starved overnight then treated with 100nM Calyculin A for 30 minutes cell lysate Lane 8: PC-12 cell lysate Lane 9: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723662) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of HeLa cells untreated (left) / treated with 100nM Calyculin A for 30 minutes (right) labeling Phospho-TAK1 (S412). Cells were fixed and permeabilized. Then stained with the primary antibody (HA723662, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Flow cytometric analysis of NIH/3T3 cells untreated (left) / treated with 100nM Calyculin A for 30 minutes (right) labeling Phospho-TAK1 (S412). Cells were fixed and permeabilized. Then stained with the primary antibody (HA723662, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier

TAK1
HUABIO
catalog: HA750424

domestic rabbit monoclonal (JM73-19)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of TAK1 on different lysates with Rabbit anti-TAK1 antibody (HA750424) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD TAK1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 67 kDa Observed band size: 72 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750424) at 1/1,000 dilution was used in 2% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of TAK1 on different lysates with Rabbit anti-TAK1 antibody (HA750424) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Mouse lung tissue lysate (40 µg/Lane) Lane 6: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 67 kDa Observed band size: 72 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of TAK1 on different lysates with Rabbit anti-TAK1 antibody (HA750424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-TAK1 (S412)
HUABIO
catalog: HA751525

domestic rabbit monoclonal (PSH14-64)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of Phospho-TAK1 (S412) on different lysates with Rabbit anti-Phospho-TAK1 (S412) antibody (HA751525) at 1/5,000 dilution. Lane 1: K-562 cell lysate Lane 2: C2C12 cell lysate Lane 3: C6 cell lysate Lane 4: HeLa cell lysate Lane 5: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: NIH/3T3 starved overnight then treated with 100nM Calyculin A for 30 minutes cell lysate Lane 8: PC-12 cell lysate Lane 9: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751525) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of HeLa cells untreated (left) / treated with 100nM Calyculin A for 30 minutes (right) labeling Phospho-TAK1 (S412). Cells were fixed and permeabilized. Then stained with the primary antibody (HA751525, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Flow cytometric analysis of NIH/3T3 cells untreated (left) / treated with 100nM Calyculin A for 30 minutes (right) labeling Phospho-TAK1 (S412). Cells were fixed and permeabilized. Then stained with the primary antibody (HA751525, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier