domestic rabbit monoclonal (JF1-045)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of CD147 on different lysates with Rabbit anti-CD147 antibody (ET1702-58) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane) Lane 2: NCI-H226 cell lysate (15 µg/Lane) Lane 3: 786-0 cell lysate (15 µg/Lane) Lane 4: RAW264.7 cell lysate (15 µg/Lane) Lane 5: Mouse liver tissue lysate (20 µg/Lane) Lane 6: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 42 kDa Observed band size: 45-60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-58) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
All lanes: Western blot analysis of CD147 with anti-CD147 antibody[JF1-045] (ET1702-58) at 1:500 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2/3: CD147 knockdown Hela whole cell lysate (10 µg). ET1702-58 was shown to specifically react with CD147 in wild-type Hela cells. Weakened bands were observed when CD147 knockdown samples were tested. Wild-type and CD147 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-58, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ICC staining of CD147 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 50μl
price: 205.00 USD
to the supplier
domestic rabbit monoclonal (PSH13-06)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human CD147 matched pair antibodies Capture: HA725085, Human CD147 Rabbit mAb [PSH13-06] Detector: HA725086, Human CD147 Rabbit mAb [PSH13-07] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725085) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD147 protein (HA210959) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725086, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CD147 in human urine samples. Capture: HA725085, Human CD147 Rabbit mAb [PSH13-06] Detector: HA725086, Human CD147 Rabbit mAb [PSH13-07] The concentrations of CD147 were measured in duplicates, interpolated from the CD147 standard curve and corrected for sample dilution. Undiluted samples are human urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD147 concentration was determined to be 493 pg/ml in human urine.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH13-07)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human CD147 matched pair antibodies Capture: HA725085, Human CD147 Rabbit mAb [PSH13-06] Detector: HA725086, Human CD147 Rabbit mAb [PSH13-07] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725085) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD147 protein (HA210959) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725086, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CD147 in human urine samples. Capture: HA725085, Human CD147 Rabbit mAb [PSH13-06] Detector: HA725086, Human CD147 Rabbit mAb [PSH13-07] The concentrations of CD147 were measured in duplicates, interpolated from the CD147 standard curve and corrected for sample dilution. Undiluted samples are human urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD147 concentration was determined to be 493 pg/ml in human urine.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH13-07)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of human CD147 matched pair antibodies Capture: HA725085, Human CD147 Rabbit mAb [PSH13-06] Detector: HA725086, Human CD147 Rabbit mAb [PSH13-07] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725085) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD147 protein (HA210959) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725086, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CD147 in human urine samples. Capture: HA725085, Human CD147 Rabbit mAb [PSH13-06] Detector: HA725086, Human CD147 Rabbit mAb [PSH13-07] The concentrations of CD147 were measured in duplicates, interpolated from the CD147 standard curve and corrected for sample dilution. Undiluted samples are human urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD147 concentration was determined to be 493 pg/ml in human urine.
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit monoclonal (JF1-045)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section