domestic rabbit monoclonal (JF10-00)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: Cerebral cortex (P0) Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required
Application: IHC-Fr Species: Mouse Site: E14.5 embryonic brain Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
Fluorescence multiplex immunohistochemical analysis of mouse brain (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-NeuN (ET1602-12, red), anti-PAX6 (ET1612-58, green), anti-CD34 (ET1606-11, gray), anti-MAP2 (HA500177, magenta) and anti-TBR1 (ET1702-97, yellow) on mouse brain. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in five rounds of staining: in the order of ET1602-12 (1/5,000 dilution), ET1612-58 (1/1,000 dilution), ET1606-11 (1/2,000 dilution), HA500177 (1/5,000 dilution) and ET1702-97 (1/1,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (JF10-00)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: Cerebral cortex (P0) Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required
Application: IHC-Fr Species: Mouse Site: E14.5 embryonic brain Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
Application: IF-tissue Species: Mouse Site: E14.5 embryonic brain Sample: Paraffin-embedded section Antibody concentration: 1:500
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit polyclonal (JF10-00)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: Cerebral cortex (P0) Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
Immunohistochemical analysis of paraffin-embedded E14.5 rat embryo tissue with Rabbit anti-TBR1 antibody (HA601422) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601422) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-TBR1 antibody (HA601422) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601422) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit polyclonal (JF10-00)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: Cerebral cortex (P0) Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
Immunohistochemical analysis of paraffin-embedded E14.5 rat embryo tissue with Rabbit anti-TBR1 antibody (HA610262) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610262) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-TBR1 antibody (HA610262) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610262) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier