SOX9
HUABIO
catalog: ET1611-56-50UL

domestic rabbit monoclonal (SN74-09)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of SOX9 on different lysates with Rabbit anti-SOX9 antibody (ET1611-56) at 1/10,000 dilution and competitor's antibody at 1/5,000 dilution. Lane 1: SW480 cell lysate Lane 2: MDA-MB-231 cell lysate Lane 3: MCF7 cell lysate (negative) Lane 4: PANC-1 cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 56 kDa Observed band size: 70 kDa Exposure time: 1 minute 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-56) at 1/10,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of SOX9 on different lysates with Rabbit anti-SOX9 antibody (ET1611-56) at 1/10,000 dilution. Lane 1: MDA-MB-231-si NT cell lysate Lane 2: MDA-MB-231-si SOX9 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 56 kDa Observed band size: 70 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-56) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fluorescence multiplex immunohistochemical analysis of mouse liver (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-β-catenin (ET1601-5, Tangerine), anti-αSMA (ET1607-53, Yellow), anti-SOX9 (ET1611-56, Green), anti-Albumin (ET1702-55, Cyan) anti-GS (EM1902-39, Magenta) and anti-CK19 (ET1601-6, Orange) on mouse liver. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in six rounds of staining: in the order of ET1601-5 (1/2,000 dilution), ET1607-53 (1/3,000 dilution), ET1611-56 (1/1,500 dilution), ET1702-55 (1/3,000 dilution), EM1902-39 (1/2,000 dilution) and ET1601-6 (1/3,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 50μl
price: 205.00 USD
to the supplier

SOX9
HUABIO
catalog: HA723548

domestic rabbit monoclonal (PSH13-59)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-SOX9 antibody (HA723548) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723548) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

SOX9
HUABIO
catalog: HA750262

domestic rabbit monoclonal (SN74-09)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of SOX9 on different lysates with Rabbit anti-SOX9 antibody (HA750262) at 1/10,000 dilution and competitor's antibody at 1/5,000 dilution. Lane 1: SW480 cell lysate Lane 2: MDA-MB-231 cell lysate Lane 3: MCF7 cell lysate (negative) Lane 4: PANC-1 cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 56 kDa Observed band size: 70 kDa Exposure time: 1 minute 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750262) at 1/10,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of SOX9 on different lysates with Rabbit anti-SOX9 antibody (HA750262) at 1/10,000 dilution. Lane 1: MDA-MB-231-si NT cell lysate Lane 2: MDA-MB-231-si SOX9 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 56 kDa Observed band size: 70 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750262) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-SOX9 antibody (HA750262) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750262) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

SOX9
HUABIO
catalog: HA751478

domestic rabbit monoclonal (PSH13-59)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-SOX9 antibody (HA751478) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751478) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

SOX9
HUABIO
catalog: IRS199RB

domestic rabbit monoclonal
reactivity: human

mIHC analysis of human liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-SOX9 antibody (IRS199RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

mIHC analysis of human colon tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-SOX9 antibody (IRS199RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 50T
price: 278.00 USD
to the supplier

SOX9
HUABIO
catalog: IRS279MS

domestic rabbit polyclonal
reactivity: human

mIHC analysis of human intestine tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-SOX9 antibody (IRS279MS) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 100μl
price: 279.00 USD
to the supplier

SOX9
HUABIO
catalog: IRS280RT

domestic rabbit polyclonal
reactivity: human

mIHC analysis of human kidney tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rat anti-SOX9 antibody (IRS280RT) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900810). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 100μl
price: 279.00 USD
to the supplier