alpha+beta Synuclein
HUABIO
catalog: ET1609-66

domestic rabbit monoclonal (ST05-21)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of alpha+beta Synuclein on different lysates with Rabbit anti-alpha+beta Synuclein antibody (ET1609-66) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 14 kDa Observed band size: 15 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-66) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

ICC staining of alpha+beta Synuclein in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-66, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of alpha+beta Synuclein in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-66, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-alpha Synuclein (S129)
HUABIO
catalog: ET7107-30-50UL

domestic rabbit monoclonal (JB22-44)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Immunocytochemistry analysis of HeLa cells labeling Phospho-alpha Synuclein (S129) with Rabbit anti-Phospho-alpha Synuclein (S129) antibody (ET7107-30) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-alpha Synuclein (S129) antibody (ET7107-30) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of Neuro-2a cells labeling Phospho-alpha Synuclein (S129) with Rabbit anti-Phospho-alpha Synuclein (S129) antibody (ET7107-30) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-alpha Synuclein (S129) antibody (ET7107-30) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of C6 cells labeling Phospho-alpha Synuclein (S129) with Rabbit anti-Phospho-alpha Synuclein (S129) antibody (ET7107-30) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-alpha Synuclein (S129) antibody (ET7107-30) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 50μl
price: 205.00 USD
to the supplier

Alpha-Synuclein
HUABIO
catalog: ET7107-31

domestic rabbit monoclonal (JB42-33)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Alpha-Synuclein on different lysates with Rabbit anti-Alpha-Synuclein antibody (ET7107-31) at 1/2,000 dilution. Lane 1: SK-MEL-28 cell lysate (20 µg/Lane) Lane 2: Human brain tissue lysate (40 µg/Lane) Predicted band size: 14 kDa Observed band size: 18 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-31) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Alpha-Synuclein antibody (ET7107-31) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-31) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-Alpha-Synuclein antibody (ET7107-31) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-31) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Alpha-Synuclein
HUABIO
catalog: HA723035

domestic rabbit monoclonal (PSH08-83)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

Western blot analysis of Alpha-Synuclein on different lysates with Rabbit anti-Alpha-Synuclein antibody (HA723035) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with Alpha-Synuclein cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 14 kDa Observed band size: 25 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723035) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Human Alpha-Synuclein
HUABIO
catalog: HA723467

domestic rabbit monoclonal (JB42-33)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Alpha-Synuclein matched pair antibodies Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33] Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723467) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human Alpha-synuclein protein (HA211150) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723468, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native alpha-Synuclein in SH-SY5Y extract samples based on a 1,000 µg/ml extract load. Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33] Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56] The concentrations of alpha-Synuclein were measured in duplicates, interpolated from the alpha-Synuclein standard curve and corrected for sample dilution. Undiluted samples are SH-SY5Y extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-Synuclein concentration was determined to be 2,195 pg/ml in SH-SY5Y extract.

Interpolated concentrations of native alpha-Synuclein in human serum samples. Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33] Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56] The concentrations of alpha-Synuclein were measured in duplicates, interpolated from the alpha-Synuclein standard curve and corrected for sample dilution. Undiluted samples are human serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-Synuclein concentration was determined to be 21.2 ng/ml in human serum.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Alpha-Synuclein
HUABIO
catalog: HA723468

domestic rabbit monoclonal (PSH12-56)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Alpha-Synuclein matched pair antibodies Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33] Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723467) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human Alpha-synuclein protein (HA211150) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723468, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native alpha-Synuclein in SH-SY5Y extract samples based on a 1,000 µg/ml extract load. Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33] Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56] The concentrations of alpha-Synuclein were measured in duplicates, interpolated from the alpha-Synuclein standard curve and corrected for sample dilution. Undiluted samples are SH-SY5Y extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-Synuclein concentration was determined to be 2,195 pg/ml in SH-SY5Y extract.

Interpolated concentrations of native alpha-Synuclein in human serum samples. Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33] Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56] The concentrations of alpha-Synuclein were measured in duplicates, interpolated from the alpha-Synuclein standard curve and corrected for sample dilution. Undiluted samples are human serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-Synuclein concentration was determined to be 21.2 ng/ml in human serum.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Alpha-Synuclein
HUABIO
catalog: HA723469B

domestic rabbit monoclonal (PSH12-56)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human Alpha-Synuclein matched pair antibodies Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33] Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723467) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human Alpha-synuclein protein (HA211150) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723468, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native alpha-Synuclein in SH-SY5Y extract samples based on a 1,000 µg/ml extract load. Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33] Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56] The concentrations of alpha-Synuclein were measured in duplicates, interpolated from the alpha-Synuclein standard curve and corrected for sample dilution. Undiluted samples are SH-SY5Y extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-Synuclein concentration was determined to be 2,195 pg/ml in SH-SY5Y extract.

Interpolated concentrations of native alpha-Synuclein in human serum samples. Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33] Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56] The concentrations of alpha-Synuclein were measured in duplicates, interpolated from the alpha-Synuclein standard curve and corrected for sample dilution. Undiluted samples are human serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-Synuclein concentration was determined to be 21.2 ng/ml in human serum.
quantity: 100μl
price: 409.00 USD
to the supplier

Alpha-Synuclein
HUABIO
catalog: HA751248

domestic rabbit monoclonal (PSH08-83)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

Western blot analysis of Alpha-Synuclein on different lysates with Rabbit anti-Alpha-Synuclein antibody (HA751248) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with Alpha-Synuclein cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 14 kDa Observed band size: 25 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751248) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

Anti-Alpha Synuclein Antibody [JB42-33]
HUABIO
catalog: ET7107-31TR

domestic rabbit monoclonal (JB42-33)
reactivity: human, mouse
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

quantity: 20 uL
price: 99 USD
to the supplier