domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (HA500539) at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500539) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Neuro-2a cells labeling SNAP25 with Rabbit anti-SNAP25 antibody (HA500539) at 1/5,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SNAP25 antibody (HA500539) at 1/5,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SNAP25 antibody (HA500539) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500539) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit monoclonal (JE45-86)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (HA721892) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: Neuro-2a cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (negative) (20 µg/Lane) Lane 4: Human brain tissue lysate (40 µg/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721892) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of SH-SY5Y cells labeling SNAP25 with Rabbit anti-SNAP25 antibody (HA721892) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SNAP25 antibody (HA721892) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SNAP25 antibody (HA721892) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721892) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH17-65)
reactivity: human, mouse
application: ELISA
reactivity: human, mouse
application: ELISA
Sandwich ELISA analysis of Human SNAP25 matched pair antibodies Capture: HA725227, SNAP25 Rabbit mAb [PSH17-65] Detector: HA725228, SNAP25 Rabbit mAb [PSH17-66] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725227) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human SNAP25 protein (HA211155) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725228, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native SNAP25 in SH-SY5Y and PANC-1 extract samples based on a 1,000 μg/ml extract load. Capture: HA725227, SNAP25 Rabbit mAb [PSH17-65] Detector: HA725228, SNAP25 Rabbit mAb [PSH17-66] The concentrations of SNAP25 were measured in duplicates, interpolated from the SNAP25 standard curve and corrected for sample dilution. Undiluted samples are SH-SY5Y extract 13% and PANC-1 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SNAL25 concentration was determined to be 2,561 pg/ml in SH-SY5Y extract and undetectable in PANC-1 extract.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH17-66)
reactivity: human, mouse
application: ELISA
reactivity: human, mouse
application: ELISA
Sandwich ELISA analysis of Human SNAP25 matched pair antibodies Capture: HA725227, SNAP25 Rabbit mAb [PSH17-65] Detector: HA725228, SNAP25 Rabbit mAb [PSH17-66] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725227) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human SNAP25 protein (HA211155) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725228, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native SNAP25 in SH-SY5Y and PANC-1 extract samples based on a 1,000 μg/ml extract load. Capture: HA725227, SNAP25 Rabbit mAb [PSH17-65] Detector: HA725228, SNAP25 Rabbit mAb [PSH17-66] The concentrations of SNAP25 were measured in duplicates, interpolated from the SNAP25 standard curve and corrected for sample dilution. Undiluted samples are SH-SY5Y extract 13% and PANC-1 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SNAL25 concentration was determined to be 2,561 pg/ml in SH-SY5Y extract and undetectable in PANC-1 extract.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH17-66)
reactivity: human, mouse
conjugate: biotin
application: ELISA
reactivity: human, mouse
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Human SNAP25 matched pair antibodies Capture: HA725227, SNAP25 Rabbit mAb [PSH17-65] Detector: HA725228, SNAP25 Rabbit mAb [PSH17-66] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725227) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human SNAP25 protein (HA211155) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725228, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native SNAP25 in SH-SY5Y and PANC-1 extract samples based on a 1,000 μg/ml extract load. Capture: HA725227, SNAP25 Rabbit mAb [PSH17-65] Detector: HA725228, SNAP25 Rabbit mAb [PSH17-66] The concentrations of SNAP25 were measured in duplicates, interpolated from the SNAP25 standard curve and corrected for sample dilution. Undiluted samples are SH-SY5Y extract 13% and PANC-1 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SNAL25 concentration was determined to be 2,561 pg/ml in SH-SY5Y extract and undetectable in PANC-1 extract.
quantity: 100μl
price: 385.00 USD
to the supplier