domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Hamartin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500199, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: LO2 cell lysate Lane 2: 293 cell lysate Lane 3: A431 cell lysate
Western blot analysis of Hamartin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500199, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse pancreas tissue lysate Lane 2: Rat brain tissue lysate
ICC staining of Hamartin in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500199, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 330 USD
to the supplier
mouse monoclonal (A6F1)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Hamartin on different lysates with Mouse anti-Hamartin antibody (HA600004) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: 293 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 130 kDa Observed band size: 145 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600004) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Hamartin antibody (HA600004) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600004) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue with Mouse anti-Hamartin antibody (HA600004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600004) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-67)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
Western blot analysis of Hamartin on different lysates with Rabbit anti-Hamartin antibody (HA723665) at 1/20,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: COS-1 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: MEF cell lysate Lane 6: C2C12 cell lysate Lane 7: PC-12 cell lysate Lane 8: C6 cell lysate Lane 9: Mouse brain tissue lysate Lane 10: Mouse heart tissue lysate Lane 11: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 130 kDa Observed band size: 140 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723665) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Hamartin was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with HA723665 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723665 at 1/20,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 cell lysate (input) Lane 2: HA723665 IP in NIH/3T3 cell lysate Lane 3: Rabbit IgG instead of HA723665 in NIH/3T3 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 50 seconds; ECL: K1801
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-67)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
Western blot analysis of Hamartin on different lysates with Rabbit anti-Hamartin antibody (HA751528) at 1/20,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: COS-1 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: MEF cell lysate Lane 6: C2C12 cell lysate Lane 7: PC-12 cell lysate Lane 8: C6 cell lysate Lane 9: Mouse brain tissue lysate Lane 10: Mouse heart tissue lysate Lane 11: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 130 kDa Observed band size: 140 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751528) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Hamartin was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with HA751528 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751528 at 1/20,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 cell lysate (input) Lane 2: HA751528 IP in NIH/3T3 cell lysate Lane 3: Rabbit IgG instead of HA751528 in NIH/3T3 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 50 seconds; ECL: K1801
quantity: 100μl
price: 649.00 USD
to the supplier