domestic rabbit monoclonal (JM10-95)
reactivity: human
application: western blot, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of CD62P on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HUVEC cell lysate Lane 2: Human placenta tissue lysate Predicted band size: 91 kDa Observed band size: 100 kDa
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD62P antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD62P antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-70)
reactivity: human, mouse, rat
application: ELISA
reactivity: human, mouse, rat
application: ELISA
Sandwich ELISA analysis of Human P-Selectin matched pair antibodies Capture: HA723872, P-Selectin / CD62P Rabbit mAb [PSH16-70] Detector: HA723873, P-Selectin / CD62P Rabbit mAb [PSH16-71] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723872) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human P-Selectin / CD62P protein (HA211064) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723873, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Sandwich ELISA analysis of mouse P-Selectin matched pair antibodies Capture: HA723872, P-Selectin / CD62P Rabbit mAb [PSH16-70] Detector: HA723873, P-Selectin / CD62P Rabbit mAb [PSH16-71] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723872) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse P-Selectin / CD62P protein (HA211140) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723873, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native P-Selectin in human serum samples and HeLa cell culture supernatant. Capture: HA723872, P-Selectin / CD62P Rabbit mAb [PSH16-70] Detector: HA723873, P-Selectin / CD62P Rabbit mAb [PSH16-71] The concentrations of P-Selectin were measured in triplicates, interpolated from the P-Selectin standard curve and corrected for sample dilution. Undiluted samples are human serum 5% and HeLa cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean P-Selectin concentration was determined to be 95,663 pg/ml in human serum and undetectable in HeLa cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-71)
reactivity: human, mouse, rat
application: ELISA
reactivity: human, mouse, rat
application: ELISA
Sandwich ELISA analysis of Human P-Selectin matched pair antibodies Capture: HA723872, P-Selectin / CD62P Rabbit mAb [PSH16-70] Detector: HA723873, P-Selectin / CD62P Rabbit mAb [PSH16-71] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723872) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human P-Selectin / CD62P protein (HA211064) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723873, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Sandwich ELISA analysis of mouse P-Selectin matched pair antibodies Capture: HA723872, P-Selectin / CD62P Rabbit mAb [PSH16-70] Detector: HA723873, P-Selectin / CD62P Rabbit mAb [PSH16-71] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723872) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse P-Selectin / CD62P protein (HA211140) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723873, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native P-Selectin in human serum samples and HeLa cell culture supernatant. Capture: HA723872, P-Selectin / CD62P Rabbit mAb [PSH16-70] Detector: HA723873, P-Selectin / CD62P Rabbit mAb [PSH16-71] The concentrations of P-Selectin were measured in triplicates, interpolated from the P-Selectin standard curve and corrected for sample dilution. Undiluted samples are human serum 5% and HeLa cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean P-Selectin concentration was determined to be 95,663 pg/ml in human serum and undetectable in HeLa cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-71)
reactivity: human, mouse, rat
conjugate: biotin
application: ELISA
reactivity: human, mouse, rat
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Human P-Selectin matched pair antibodies Capture: HA723872, P-Selectin / CD62P Rabbit mAb [PSH16-70] Detector: HA723873, P-Selectin / CD62P Rabbit mAb [PSH16-71] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723872) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human P-Selectin / CD62P protein (HA211064) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723873, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Sandwich ELISA analysis of mouse P-Selectin matched pair antibodies Capture: HA723872, P-Selectin / CD62P Rabbit mAb [PSH16-70] Detector: HA723873, P-Selectin / CD62P Rabbit mAb [PSH16-71] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723872) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse P-Selectin / CD62P protein (HA211140) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723873, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native P-Selectin in human serum samples and HeLa cell culture supernatant. Capture: HA723872, P-Selectin / CD62P Rabbit mAb [PSH16-70] Detector: HA723873, P-Selectin / CD62P Rabbit mAb [PSH16-71] The concentrations of P-Selectin were measured in triplicates, interpolated from the P-Selectin standard curve and corrected for sample dilution. Undiluted samples are human serum 5% and HeLa cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean P-Selectin concentration was determined to be 95,663 pg/ml in human serum and undetectable in HeLa cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier