Human CD62L
HUABIO
catalog: HA723575
domestic rabbit monoclonal (PSH13-84)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human CD62L matched pair antibodies Capture: HA723575, Human CD62L Rabbit mAb [PSH13-84] Detector: HA723576, Human CD62L Rabbit mAb [PSH13-85] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723575) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD62L protein (HA211181) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723576, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD62L in human samples. Capture: HA723575, Human CD62L Rabbit mAb [PSH13-84] Detector: HA723576, Human CD62L Rabbit mAb [PSH13-85] Interpolated concentration of native CD62L was measured in duplicate at different sample concentrations and interpolated from the CD62L standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD62L concentration was determined to be 6,425 pg/mL in Jurkat Cell Supernatant. There was no detectable signal in Hela cell supernatant.

Interpolated concentrations of spiked CD62L in cell culture media samples. Capture: HA723575, Human CD62L Rabbit mAb [PSH13-84] Detector: HA723576, Human CD62L Rabbit mAb [PSH13-85] The concentrations of CD62L were measured in duplicates, interpolated from the CD62L standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human CD62L
HUABIO
catalog: HA723576
domestic rabbit monoclonal (PSH13-85)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human CD62L matched pair antibodies Capture: HA723575, Human CD62L Rabbit mAb [PSH13-84] Detector: HA723576, Human CD62L Rabbit mAb [PSH13-85] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723575) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD62L protein (HA211181) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723576, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of human CD62L matched pair antibodies Capture: HA723576, Human CD62L Rabbit mAb [PSH13-85] Detector: HA723578, Human CD62L Rabbit mAb [PSH13-86] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723576) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD62L protein (HA211181) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723578, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD62L in human samples. Capture: HA723575, Human CD62L Rabbit mAb [PSH13-84] Detector: HA723576, Human CD62L Rabbit mAb [PSH13-85] Interpolated concentration of native CD62L was measured in duplicate at different sample concentrations and interpolated from the CD62L standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD62L concentration was determined to be 6,425 pg/mL in Jurkat Cell Supernatant. There was no detectable signal in Hela cell supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
Human CD62L
HUABIO
catalog: HA723577B
domestic rabbit monoclonal (PSH13-85)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human CD62L matched pair antibodies Capture: HA723575, Human CD62L Rabbit mAb [PSH13-84] Detector: HA723576, Human CD62L Rabbit mAb [PSH13-85] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723575) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD62L protein (HA211181) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723576, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD62L in human samples. Capture: HA723575, Human CD62L Rabbit mAb [PSH13-84] Detector: HA723576, Human CD62L Rabbit mAb [PSH13-85] Interpolated concentration of native CD62L was measured in duplicate at different sample concentrations and interpolated from the CD62L standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD62L concentration was determined to be 6,425 pg/mL in Jurkat Cell Supernatant. There was no detectable signal in Hela cell supernatant.

Interpolated concentrations of spiked CD62L in cell culture media samples. Capture: HA723575, Human CD62L Rabbit mAb [PSH13-84] Detector: HA723576, Human CD62L Rabbit mAb [PSH13-85] The concentrations of CD62L were measured in duplicates, interpolated from the CD62L standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
Human CD62L
HUABIO
catalog: HA723578
domestic rabbit monoclonal (PSH13-86)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human CD62L matched pair antibodies Capture: HA723576, Human CD62L Rabbit mAb [PSH13-85] Detector: HA723578, Human CD62L Rabbit mAb [PSH13-86] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723576) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD62L protein (HA211181) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723578, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD62L in human samples. Capture: HA723576, Human CD62LRabbit mAb [PSH13-85] Detector: HA723578, Human CD62L Rabbit mAb [PSH13-86] Interpolated concentration of native CD62L was measured in duplicate at different sample concentrations and interpolated from the CD62L standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD62L concentration was determined to be 6,792 pg/mL in Jurkat cell supernatant. There was no detectable signal in Hela cell supernatant.

Interpolated concentrations of spiked CD62L in cell culture media samples. Capture: HA723576, Human CD62L Rabbit mAb [PSH13-85] Detector: HA723578, Human CD62L Rabbit mAb [PSH13-86] The concentrations of CD62L were measured in duplicates, interpolated from the CD62L standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human CD62L
HUABIO
catalog: HA723579B
domestic rabbit monoclonal (PSH13-86)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human CD62L matched pair antibodies Capture: HA723576, Human CD62L Rabbit mAb [PSH13-85] Detector: HA723578, Human CD62L Rabbit mAb [PSH13-86] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723576) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD62L protein (HA211181) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723578, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD62L in human samples. Capture: HA723576, Human CD62L Rabbit mAb [PSH13-85] Detector: HA723578, Human CD62L Rabbit mAb [PSH13-86] Interpolated concentration of native CD62L was measured in duplicate at different sample concentrations and interpolated from the CD62L standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD62L concentration was determined to be 6,792 pg/mL in Jurkat cell supernatant. There was no detectable signal in Hela cell supernatant.

Interpolated concentrations of spiked CD62L in cell culture media samples. Capture: HA723576, Human CD62L Rabbit mAb [PSH13-85] Detector: HA723578, Human CD62L Rabbit mAb [PSH13-86] The concentrations of CD62L were measured in duplicates, interpolated from the CD62L standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
CD62L
HUABIO
catalog: HA723694
domestic rabbit monoclonal (PSH14-92)
reactivity: human
application: flow cytometry

Flow cytometric analysis of 293T (left, negative) and Jurkat (right, positive) cells labeling CD62L. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723694, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Immunocytochemistry analysis of Jurkat (positive) and 293T (negative) labeling CD62L with Rabbit anti-CD62L antibody (HA723694) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD62L antibody (HA723694) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
CD62L
HUABIO
catalog: HA723766
domestic rabbit monoclonal (PSH15-59)
reactivity: human
application: flow cytometry

Flow cytometric analysis of human peripheral blood cells labeling CD62L. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723766, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Flow cytometric analysis of 293T (left, negative) and Jurkat (right, positive) cells labeling CD62L. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723766, 1/5,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100T
price: 169.00 USD
to the supplier
CD62L
HUABIO
catalog: HA751536
domestic rabbit monoclonal (PSH14-92)
reactivity: human
application: flow cytometry

Flow cytometric analysis of 293T (left, negative) and Jurkat (right, positive) cells labeling CD62L. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751536, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Immunocytochemistry analysis of Jurkat (positive) and 293T (negative) labeling CD62L with Rabbit anti-CD62L antibody (HA751536) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD62L antibody (HA751536) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
CD62L
HUABIO
catalog: HA751570
domestic rabbit monoclonal (PSH15-59)
reactivity: human
application: flow cytometry

Flow cytometric analysis of human peripheral blood cells labeling CD62L. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751570, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Flow cytometric analysis of 293T (left, negative) and Jurkat (right, positive) cells labeling CD62L. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751570, 1/5,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier