domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of CX3CL1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500304, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SK-BR-3 cell lysate Lane 2: HepG2 cell lysate
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CX3CL1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500304, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CX3CL1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500304, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit monoclonal (PSH16-08)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human CX3CL1 matched pair antibodies Capture: HA723813, Human CX3CL1 Rabbit mAb [PSH16-08] Detector: HA723814, Human CX3CL1 Rabbit mAb [PSH16-09] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723813) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CX3CL1protein (HA211057) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA723814, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CX3CL1 in HUVEC cell culture supernatant untreated or treated with TNFα and IFNγ for 24h. Capture: HA723813, Human CX3CL1 Rabbit mAb [PSH16-08] Detector: HA723814, Human CX3CL1 Rabbit mAb [PSH16-09] Interpolated concentration of native CX3CL1 was measured in duplicate at different sample concentrations and interpolated from the CX3CL1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL3 concentration was determined to be 9109 pg/ml in HUVEC treated cell culture supernatant, undetectable in HUVEC untreated cell culture supernatant.
Interpolated concentrations of spiked CX3CL1 in cell culture media samples. Capture: HA723813, Human CX3CL1 Rabbit mAb [PSH16-08] Detector: HA723814, Human CX3CL1 Rabbit mAb [PSH16-09] The concentrations of CX3CL1 were measured in duplicates, interpolated from the CX3CL1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-09)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human CX3CL1 matched pair antibodies Capture: HA723813, Human CX3CL1 Rabbit mAb [PSH16-08] Detector: HA723814, Human CX3CL1 Rabbit mAb [PSH16-09] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723813) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CX3CL1protein (HA211057) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA723814, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CX3CL1 in HUVEC cell culture supernatant untreated or treated with TNFα and IFNγ for 24h. Capture: HA723813, Human CX3CL1 Rabbit mAb [PSH16-08] Detector: HA723814, Human CX3CL1 Rabbit mAb [PSH16-09] Interpolated concentration of native CX3CL1 was measured in duplicate at different sample concentrations and interpolated from the CX3CL1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL3 concentration was determined to be 9109 pg/ml in HUVEC treated cell culture supernatant, undetectable in HUVEC untreated cell culture supernatant.
Interpolated concentrations of spiked CX3CL1 in cell culture media samples. Capture: HA723813, Human CX3CL1 Rabbit mAb [PSH16-08] Detector: HA723814, Human CX3CL1 Rabbit mAb [PSH16-09] The concentrations of CX3CL1 were measured in duplicates, interpolated from the CX3CL1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-09)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Human CX3CL1 matched pair antibodies Capture: HA723813, Human CX3CL1 Rabbit mAb [PSH16-08] Detector: HA723814, Human CX3CL1 Rabbit mAb [PSH16-09] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723813) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CX3CL1protein (HA211057) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA723814, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CX3CL1 in HUVEC cell culture supernatant untreated or treated with TNFα and IFNγ for 24h. Capture: HA723813, Human CX3CL1 Rabbit mAb [PSH16-08] Detector: HA723814, Human CX3CL1 Rabbit mAb [PSH16-09] Interpolated concentration of native CX3CL1 was measured in duplicate at different sample concentrations and interpolated from the CX3CL1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL3 concentration was determined to be 9109 pg/ml in HUVEC treated cell culture supernatant, undetectable in HUVEC untreated cell culture supernatant.
Interpolated concentrations of spiked CX3CL1 in cell culture media samples. Capture: HA723813, Human CX3CL1 Rabbit mAb [PSH16-08] Detector: HA723814, Human CX3CL1 Rabbit mAb [PSH16-09] The concentrations of CX3CL1 were measured in duplicates, interpolated from the CX3CL1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier