CCL3 / MIP-1 alpha
HUABIO
catalog: HA72$3309

domestic rabbit monoclonal (PSH11-27)
reactivity: human
application: western blot, flow cytometry

quantity: 100μl
price: 385.00 USD
to the supplier

CCL3 / MIP-1 alpha
HUABIO
catalog: HA723310

domestic rabbit monoclonal (PSH11-28)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CCL3 / MIP-1 alpha on different lysates with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA723310) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723310) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CCL3 / MIP-1 alpha on different lysates with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA723310) at 1/2,000 dilution. Lane 1: NK-92 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (negative) (20 µg/Lane) Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723310) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of RAW264.7 cells untreated / treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours labeling CCL3 / MIP-1 alpha with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA723310) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA723310) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier

Human CCL3 / MIP-1 alpha
HUABIO
catalog: HA723377

domestic rabbit monoclonal (PSH11-76)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human CCL3 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723377) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL3 / MIP-1 alpha protein (HA210901) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA723378, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CLL3 in human THP-1 supernatant treated or untreated with PMA and LPS. Interpolated concentration of native CLL3 was measured in duplicate at different sample concentrations and interpolated from the CLL3 standard curves. Undiluted samples are as follows: untreated 100% and treated 12.5%. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CLL3 concentration was determined to be 27 pg/ml in untreated THP-1 cell culture supernatant and 5,007 pg/mL in treated THP-1 supernatant.

Interpolated concentrations of spiked CLL3 in cell culture media samples. The concentrations of CLL3 were measured in duplicates, interpolated from the CLL3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Human CCL3 / MIP-1 alpha
HUABIO
catalog: HA723378

domestic rabbit monoclonal (PSH11-77)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human CCL3 matched pair antibodies Capture: HA723377, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-76] Detector: HA723378, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-77] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723377) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL3 / MIP-1 alpha protein (HA210901) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA723378, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of human CCL3 matched pair antibodies Capture: HA723380, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-78] Detector: HA723378, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-77] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723380) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL3 / MIP-1 alpha protein (HA210901) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA723378, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CLL3 in human THP-1 supernatant treated or untreated with PMA and LPS. Capture: HA723377, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-76] Detector: HA723378, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-77] Interpolated concentration of native CLL3 was measured in duplicate at different sample concentrations and interpolated from the CLL3 standard curves. Undiluted samples are as follows: untreated 100% and treated 12.5%. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CLL3 concentration was determined to be 27 pg/ml in untreated THP-1 cell culture supernatant and 5,007 pg/mL in treated THP-1 supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Human CCL3 / MIP-1 alpha
HUABIO
catalog: HA723379B

domestic rabbit monoclonal (PSH11-77)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human CCL3 matched pair antibodies Capture: HA723377, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-76] Detector: HA723378, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-77] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723377) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL3 / MIP-1 alpha protein (HA210901) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA723379B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of human CCL3 matched pair antibodies Capture: HA723380, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-78] Detector: HA723378, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-77] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723380) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL3 / MIP-1 alpha protein (HA210901) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA723379B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CLL3 in human THP-1 supernatant treated or untreated with PMA and LPS. Capture: HA723377, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-76] Detector: HA723378, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-77] Interpolated concentration of native CLL3 was measured in duplicate at different sample concentrations and interpolated from the CLL3 standard curves. Undiluted samples are as follows: untreated 100% and treated 12.5%. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CLL3 concentration was determined to be 27 pg/ml in untreated THP-1 cell culture supernatant and 5,007 pg/mL in treated THP-1 supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier

Human CCL3 / MIP-1 alpha
HUABIO
catalog: HA723380

domestic rabbit monoclonal (PSH11-78)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human CCL3 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723380) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL3 / MIP-1 alpha protein (HA210901) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA723378, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CLL3 in human THP-1 supernatant treated or untreated with PMA and LPS. Interpolated concentration of native CLL3 was measured in duplicate at different sample concentrations and interpolated from the CLL3 standard curves. Undiluted samples are as follows: untreated 100% and treated 12.5%. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CLL3 concentration was determined to be 25 pg/ml in untreated THP-1 cell culture supernatant and 5,277 pg/mL in treated THP-1 supernatant.

Interpolated concentrations of spiked CLL3 in cell culture media samples. The concentrations of CLL3 were measured in duplicates, interpolated from the CLL3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

CCL3 / MIP-1 alpha
HUABIO
catalog: HA751406

domestic rabbit monoclonal (PSH11-27)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of CCL3 / MIP-1 alpha on different lysates with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA751406) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751406) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CCL3 / MIP-1 alpha on different lysates with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA751406) at 1/2,000 dilution. Lane 1: NK-92 cell lysate Lane 2: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751406) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of NK-92 (positive) and HeLa (negative) labeling CCL3 / MIP-1 alpha with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA751406) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA751406) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier

CCL3 / MIP-1 alpha
HUABIO
catalog: HA751407

domestic rabbit monoclonal (PSH11-28)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CCL3 / MIP-1 alpha on different lysates with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA751407) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751407) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CCL3 / MIP-1 alpha on different lysates with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA751407) at 1/2,000 dilution. Lane 1: NK-92 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (negative) (20 µg/Lane) Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751407) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of RAW264.7 cells untreated / treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours labeling CCL3 / MIP-1 alpha with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA751407) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA751407) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier

CCL3 / MIP-1 alpha
HUABIO
catalog: R1510-10

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of CCL3 / MIP-1 alpha on different lysates with Rabbit anti-CCL3 / MIP-1 alpha antibody (R1510-10) at 1/1,000 dilution. Lane 1: NK-92 cell lysate Lane 2: THP-1 cell lysate Lane 3: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier

CCL3 / MIP-1 alpha
HUABIO
catalog: R1510-9

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: ELISA, flow cytometry, immunohistochemistry - paraffin section

Immunocytochemical staining of PANC-1 cells using anti-CCL3 rabbit polyclonal antibody.

Immunocytochemical staining of A549 cells using anti-CCL3 rabbit polyclonal antibody.

Immunohistochemical analysis of paraffin- embedded mouse lung tissue using anti-CCL3 rabbit polyclonal antibody.
quantity: 100μl
price: 330 USD
to the supplier