domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of CCL2 / MCP-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500042, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: Hela cell lysate
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-CCL2 / MCP-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500042, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-CCL2 / MCP-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500042, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit monoclonal (PSH18-21)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human CCL2(MCP1) matched pair antibodies Capture: HA725195, Human CCL2 / MCP-1 Rabbit mAb [PSH18-21] Detector: HA725196, Human CCL2 / MCP-1 Rabbit mAb [PSH18-22] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725195) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL2 / MCP-1 protein (HA210787) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725196, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CCL2(MCP1) in stimulated and unstimulated THP-1 extract samples based on a 1,000 µg/ml extract load. Capture: HA725195, Human CCL2 / MCP-1 Rabbit mAb [PSH18-21] Detector: HA725196, Human CCL2 / MCP-1 Rabbit mAb [PSH18-22] THP-1 cells were treated with 80nM PMA for 24h then treated with 100ng/ml LPS for 4h then together with 1μg/ml BFA for another 3h. The concentrations of CCL2(MCP1) were measured in duplicates, interpolated from the CCL2(MCP1) standard curve and corrected for sample dilution. Undiluted samples are stimulated and unstimulated 33%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL2(MCP1) concentration was determined to be 1,287 pg/ml in stimulated and 207 pg/ml in unstimulated.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH18-22)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human CCL2(MCP1) matched pair antibodies Capture: HA725195, Human CCL2 / MCP-1 Rabbit mAb [PSH18-21] Detector: HA725196, Human CCL2 / MCP-1 Rabbit mAb [PSH18-22] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725195) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL2 / MCP-1 protein (HA210787) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725196, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CCL2(MCP1) in stimulated and unstimulated THP-1 extract samples based on a 1,000 µg/ml extract load. Capture: HA725195, Human CCL2 / MCP-1 Rabbit mAb [PSH18-21] Detector: HA725196, Human CCL2 / MCP-1 Rabbit mAb [PSH18-22] THP-1 cells were treated with 80nM PMA for 24h then treated with 100ng/ml LPS for 4h then together with 1μg/ml BFA for another 3h. The concentrations of CCL2(MCP1) were measured in duplicates, interpolated from the CCL2(MCP1) standard curve and corrected for sample dilution. Undiluted samples are stimulated and unstimulated 33%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL2(MCP1) concentration was determined to be 1,287 pg/ml in stimulated and 207 pg/ml in unstimulated.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH18-22)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of human CCL2(MCP1) matched pair antibodies Capture: HA725195, Human CCL2 / MCP-1 Rabbit mAb [PSH18-21] Detector: HA725196, Human CCL2 / MCP-1 Rabbit mAb [PSH18-22] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725195) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL2 / MCP-1 protein (HA210787) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725196, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CCL2(MCP1) in stimulated and unstimulated THP-1 extract samples based on a 1,000 µg/ml extract load. Capture: HA725195, Human CCL2 / MCP-1 Rabbit mAb [PSH18-21] Detector: HA725196, Human CCL2 / MCP-1 Rabbit mAb [PSH18-22] THP-1 cells were treated with 80nM PMA for 24h then treated with 100ng/ml LPS for 4h then together with 1μg/ml BFA for another 3h. The concentrations of CCL2(MCP1) were measured in duplicates, interpolated from the CCL2(MCP1) standard curve and corrected for sample dilution. Undiluted samples are stimulated and unstimulated 33%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL2(MCP1) concentration was determined to be 1,287 pg/ml in stimulated and 207 pg/ml in unstimulated.
quantity: 100μl
price: 385.00 USD
to the supplier