NaV1.7
HUABIO
catalog: ER1706-44
domestic rabbit polyclonal
reactivity: human
application: flow cytometry, immunohistochemistry - paraffin section

Immunocytochemistry analysis of A549 cells labeling NaV1.7 with Rabbit anti-NaV1.7 antibody (ER1706-44) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NaV1.7 antibody (ER1706-44) at 1/500 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Immunocytochemistry analysis of HepG2 cells labeling NaV1.7 with Rabbit anti-NaV1.7 antibody (ER1706-44) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NaV1.7 antibody (ER1706-44) at 1/500 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

ICC staining NaV1.7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier
NaV1.7
HUABIO
catalog: EM1701-35
mouse monoclonal (1D6)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
quantity: 100μl
price: 360.00 USD
to the supplier