BCMA
HUABIO
catalog: HA723959

domestic rabbit monoclonal (PSH17-94)
reactivity: human
application: flow cytometry

Flow cytometric analysis of Jurkat (left, negative) and U266 (right, positive) cells labeling BCMA. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723959, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier

BCMA
HUABIO
catalog: HA723967

domestic rabbit polyclonal (PSH18-02)
reactivity: human
application: flow cytometry

Immunocytochemistry analysis of U266 (positive) and Jurkat (negative) labeling BCMA with Rabbit anti-BCMA antibody (HA723967) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BCMA antibody (HA723967) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of Jurkat (left, negative) and U266 (right, positive) cells labeling BCMA. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723967, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier

Human BCMA
HUABIO
catalog: HA725082

domestic rabbit monoclonal (PSH13-18)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human BCMA matched pair antibodies Capture: HA725082, Human BCMA Rabbit mAb [PSH13-18] Detector: HA725083, Human BCMA Rabbit mAb [PSH13-19] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725082) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human BCMA protein (HA210968) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725083, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native BCMA in human samples. Capture: HA725082, Human BCMA Rabbit mAb [PSH13-18] Detector: HA725083, Human BCMA Rabbit mAb [PSH13-19] Interpolated concentration of native BCMA was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean BCMA concentration was determined to be 10,453 pg/mL in RPMI 8226 and 351 pg/ml in human urine. There was no detectable signal in 293T cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Human BCMA
HUABIO
catalog: HA725083

domestic rabbit monoclonal (PSH13-19)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human BCMA matched pair antibodies Capture: HA725082, Human BCMA Rabbit mAb [PSH13-18] Detector: HA725083, Human BCMA Rabbit mAb [PSH13-19] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725082) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human BCMA protein (HA210968) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725083, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native BCMA in human samples. Capture: HA725082, Human BCMA Rabbit mAb [PSH13-18] Detector: HA725083, Human BCMA Rabbit mAb [PSH13-19] Interpolated concentration of native BCMA was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean BCMA concentration was determined to be 10,453 pg/mL in RPMI 8226 and 351 pg/ml in human urine. There was no detectable signal in 293T cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Human BCMA
HUABIO
catalog: HA725084B

domestic rabbit monoclonal (PSH13-19)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human BCMA matched pair antibodies Capture: HA725082, Human BCMA Rabbit mAb [PSH13-18] Detector: HA725083, Human BCMA Rabbit mAb [PSH13-19] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725082) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human BCMA protein (HA210968) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725083, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native BCMA in human samples. Capture: HA725082, Human BCMA Rabbit mAb [PSH13-18] Detector: HA725083, Human BCMA Rabbit mAb [PSH13-19] Interpolated concentration of native BCMA was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean BCMA concentration was determined to be 10,453 pg/mL in RPMI 8226 and 351 pg/ml in human urine. There was no detectable signal in 293T cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier

BCMA
HUABIO
catalog: HA751607

domestic rabbit monoclonal (PSH16-80)
reactivity: human
application: flow cytometry

Flow cytometric analysis of U266 cells labeling BCMA. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751607, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a FITC Conjugated Goat anti-rabbit IgG polyclonal Antibody (HA1124) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier