ACE2
HUABIO
catalog: ER1902-40
domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of ACE2 on human kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-40, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometric analysis of ACE2 was done on Vero cells. The cells were stained with the primary antibody (ER1902-40, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; green).
quantity: 100μl
price: 330 USD
to the supplier
ACE2
HUABIO
catalog: ET1611-58-50UL
domestic rabbit monoclonal (SN0754)
reactivity: Syrian golden hamster, human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (ET1611-58) at 1/2,000 dilution. Lane 1: Caco-2 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Mouse testis tissue lysate (40 µg/Lane) Lane 5: Mouse lung tissue lysate (40 µg/Lane) Lane 6: Rat testis tissue lysate (40 µg/Lane) Lane 7: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 92 kDa Observed band size: 130 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-58) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of ACE2 on Hamster testis (1) and stomach (2) tissue lysates using anti-ACE2 antibody at 1/1,000 dilution.

ICC staining of ACE2 in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 50μl
price: 205.00 USD
to the supplier
Human ACE2
HUABIO
catalog: HA723904
domestic rabbit monoclonal (PSH17-17)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human ACE2 matched pair antibodies Capture: HA723904, Human ACE2 Rabbit mAb [PSH17-17] Detector: HA723905, Human ACE2 Rabbit mAb [PSH17-18] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723904) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ACE2 protein (HAg2008) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723905, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native ACE2 in human serum samples and HeLa cell culture supernatant. Capture: HA723904, Human ACE2 Rabbit mAb [PSH17-17] Detector: HA723905, Human ACE2 Rabbit mAb [PSH17-18] The concentrations of ACE2 were measured in duplicates, interpolated from the ACE2 standard curve and corrected for sample dilution. Undiluted samples are human serum 50% and HeLa cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACE2 concentration was determined to be 1,633 pg/ml in human serum and undetectable in HeLa cell culture supernatant.

Interpolated concentrations of spiked ACE2 in human cell culture media samples. Capture: HA723904, Human ACE2 Rabbit mAb [PSH17-17] Detector: HA723905, Human ACE2 Rabbit mAb [PSH17-18] The concentrations of ACE2 were measured in duplicates, interpolated from the ACE2 standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human ACE2
HUABIO
catalog: HA723905
domestic rabbit monoclonal (PSH17-18)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human ACE2 matched pair antibodies Capture: HA723904, Human ACE2 Rabbit mAb [PSH17-17] Detector: HA723905, Human ACE2 Rabbit mAb [PSH17-18] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723904) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ACE2 protein (HAg2008) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723905, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native ACE2 in human serum samples and HeLa cell culture supernatant. Capture: HA723904, Human ACE2 Rabbit mAb [PSH17-17] Detector: HA723905, Human ACE2 Rabbit mAb [PSH17-18] The concentrations of ACE2 were measured in duplicates, interpolated from the ACE2 standard curve and corrected for sample dilution. Undiluted samples are human serum 50% and HeLa cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACE2 concentration was determined to be 1,633 pg/ml in human serum and undetectable in HeLa cell culture supernatant.

Interpolated concentrations of spiked ACE2 in human cell culture media samples. Capture: HA723904, Human ACE2 Rabbit mAb [PSH17-17] Detector: HA723905, Human ACE2 Rabbit mAb [PSH17-18] The concentrations of ACE2 were measured in duplicates, interpolated from the ACE2 standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human ACE2
HUABIO
catalog: HA723905B
domestic rabbit monoclonal (PSH17-18)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human ACE2 matched pair antibodies Capture: HA723904, Human ACE2 Rabbit mAb [PSH17-17] Detector: HA723905, Human ACE2 Rabbit mAb [PSH17-18] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723904) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ACE2 protein (HAg2008) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723905, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native ACE2 in human serum samples and HeLa cell culture supernatant. Capture: HA723904, Human ACE2 Rabbit mAb [PSH17-17] Detector: HA723905, Human ACE2 Rabbit mAb [PSH17-18] The concentrations of ACE2 were measured in duplicates, interpolated from the ACE2 standard curve and corrected for sample dilution. Undiluted samples are human serum 50% and HeLa cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACE2 concentration was determined to be 1,633 pg/ml in human serum and undetectable in HeLa cell culture supernatant.

Interpolated concentrations of spiked ACE2 in human cell culture media samples. Capture: HA723904, Human ACE2 Rabbit mAb [PSH17-17] Detector: HA723905, Human ACE2 Rabbit mAb [PSH17-18] The concentrations of ACE2 were measured in duplicates, interpolated from the ACE2 standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
ACE2
HUABIO
catalog: HA750264
domestic rabbit monoclonal (SN0754)
reactivity: Syrian golden hamster, human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (HA750264) at 1/2,000 dilution. Lane 1: Caco-2 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Mouse testis tissue lysate (40 µg/Lane) Lane 5: Mouse lung tissue lysate (40 µg/Lane) Lane 6: Rat testis tissue lysate (40 µg/Lane) Lane 7: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 92 kDa Observed band size: 130 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750264) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of ACE2 on Hamster testis (1) and stomach (2) tissue lysates using anti-ACE2 antibody at 1/1,000 dilution.

ICC staining of ACE2 in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750264, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 649.00 USD
to the supplier
Anti-ACE2 Antibody [SN0754]
HUABIO
catalog: ET1611-58TR
domestic rabbit monoclonal (SN0754)
reactivity: Syrian golden hamster, human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
quantity: 20 uL
price: 99 USD
to the supplier