domestic rabbit monoclonal (PSH07-19)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Retinoic Acid Receptor gamma on different lysates with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA722807) at 1/2,000 dilution. Lane 1: SK-Br-3 cell lysate (20 µg/Lane) Lane 2: T-47D cell lysate (20 µg/Lane) Lane 3: A375 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: MEF cell lysate (20 µg/Lane) Lane 6: Mouse skin tissue lysate (40 µg/Lane) Lane 7: Rat skin tissue lysate (40 µg/Lane) Predicted band size: 50 kDa Observed band size: 58 kDa Exposure time: 1 minute 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722807) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Retinoic Acid Receptor gamma on different lysates with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA722807) at 1/5,000 dilution. Lane 1: Human RARG recombinant protein, 20ng/Lane Lane 2: Human RARA recombinant protein, 20ng/Lane Lane 3: Human RARB recombinant protein, 20ng/Lane Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722807) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA722807) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722807) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH08-74)
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
Western blot analysis of Retinoic Acid Receptor alpha/beta/gamma on different lysates with Rabbit anti-Retinoic Acid Receptor alpha/beta/gamma antibody (HA723026) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: T-47D cell lysate Lane 3: A375 cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 58 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723026) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Retinoic Acid Receptor alpha/beta/gamma on different lysates with Rabbit anti-Retinoic Acid Receptor alpha/beta/gamma antibody (HA723026) at 1/1,000 dilution. Lane 1: Human RARG recombinant protein, 20ng/Lane Lane 2: Human RARA recombinant protein, 20ng/Lane Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723026) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Retinoic Acid Receptor alpha/beta/gamma on different lysates with Rabbit anti-Retinoic Acid Receptor alpha/beta/gamma antibody (HA723026) at 1/5,000 dilution. Lane 1: Human RARG recombinant protein, 20ng/Lane Lane 2: Human RARB recombinant protein, 20ng/Lane Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723026) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-19)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Retinoic Acid Receptor gamma on different lysates with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA751140) at 1/2,000 dilution. Lane 1: SK-Br-3 cell lysate (20 µg/Lane) Lane 2: T-47D cell lysate (20 µg/Lane) Lane 3: A375 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: MEF cell lysate (20 µg/Lane) Lane 6: Mouse skin tissue lysate (40 µg/Lane) Lane 7: Rat skin tissue lysate (40 µg/Lane) Predicted band size: 50 kDa Observed band size: 58 kDa Exposure time: 1 minute 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751140) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Retinoic Acid Receptor gamma on different lysates with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA751140) at 1/5,000 dilution. Lane 1: Human RARG recombinant protein, 20ng/Lane Lane 2: Human RARA recombinant protein, 20ng/Lane Lane 3: Human RARB recombinant protein, 20ng/Lane Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751140) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA751140) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751140) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH08-74)
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
Western blot analysis of Retinoic Acid Receptor alpha/beta/gamma on different lysates with Rabbit anti-Retinoic Acid Receptor alpha/beta/gamma antibody (HA751239) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: T-47D cell lysate Lane 3: A375 cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 58 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751239) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Retinoic Acid Receptor alpha/beta/gamma on different lysates with Rabbit anti-Retinoic Acid Receptor alpha/beta/gamma antibody (HA751239) at 1/1,000 dilution. Lane 1: Human RARG recombinant protein, 20ng/Lane Lane 2: Human RARA recombinant protein, 20ng/Lane Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751239) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Retinoic Acid Receptor alpha/beta/gamma on different lysates with Rabbit anti-Retinoic Acid Receptor alpha/beta/gamma antibody (HA751239) at 1/5,000 dilution. Lane 1: Human RARG recombinant protein, 20ng/Lane Lane 2: Human RARB recombinant protein, 20ng/Lane Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751239) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier