domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of RAR alpha on different lysates with Rabbit anti-RAR alpha antibody (ER1706-81) at 1/500 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-81) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ICC staining RAR alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining RAR alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit monoclonal (SN0725)
reactivity: human, mouse, rat
application: western blot, flow cytometry
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of Retinoic Acid Receptor alpha on different lysates with Rabbit anti-Retinoic Acid Receptor alpha antibody (ET1611-77) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 60 kDa Exposure time: 1 minute 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-77) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH08-74)
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
Western blot analysis of Retinoic Acid Receptor alpha/beta/gamma on different lysates with Rabbit anti-Retinoic Acid Receptor alpha/beta/gamma antibody (HA723026) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: T-47D cell lysate Lane 3: A375 cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 58 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723026) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Retinoic Acid Receptor alpha/beta/gamma on different lysates with Rabbit anti-Retinoic Acid Receptor alpha/beta/gamma antibody (HA723026) at 1/1,000 dilution. Lane 1: Human RARG recombinant protein, 20ng/Lane Lane 2: Human RARA recombinant protein, 20ng/Lane Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723026) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Retinoic Acid Receptor alpha/beta/gamma on different lysates with Rabbit anti-Retinoic Acid Receptor alpha/beta/gamma antibody (HA723026) at 1/5,000 dilution. Lane 1: Human RARG recombinant protein, 20ng/Lane Lane 2: Human RARB recombinant protein, 20ng/Lane Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723026) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH08-74)
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
Western blot analysis of Retinoic Acid Receptor alpha/beta/gamma on different lysates with Rabbit anti-Retinoic Acid Receptor alpha/beta/gamma antibody (HA751239) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: T-47D cell lysate Lane 3: A375 cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 58 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751239) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Retinoic Acid Receptor alpha/beta/gamma on different lysates with Rabbit anti-Retinoic Acid Receptor alpha/beta/gamma antibody (HA751239) at 1/1,000 dilution. Lane 1: Human RARG recombinant protein, 20ng/Lane Lane 2: Human RARA recombinant protein, 20ng/Lane Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751239) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Retinoic Acid Receptor alpha/beta/gamma on different lysates with Rabbit anti-Retinoic Acid Receptor alpha/beta/gamma antibody (HA751239) at 1/5,000 dilution. Lane 1: Human RARG recombinant protein, 20ng/Lane Lane 2: Human RARB recombinant protein, 20ng/Lane Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751239) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier