Factor XII
HUABIO
catalog: HA500586

domestic rabbit polyclonal
reactivity: mouse
application: western blot

Western blot analysis of Factor XII on different lysates with Rabbit anti-Factor XII antibody (HA500586) at 1/5,000 dilution. Lane 1: Mouse plasma lysate Lane 2: Mouse serum lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 66 kDa Observed band size: 80 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500586) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier

Mouse Factor XII
HUABIO
catalog: HA723440

domestic rabbit monoclonal (PSH12-21)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse Factor XII matched pair antibodies Capture: HA723440, Mouse Factor XII Rabbit mAb [PSH12-21] Detector: HA723441, Mouse Factor XII Rabbit mAb [PSH12-22] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723440) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Factor XII protein (HA210984) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA723441, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Factor XII in mouse samples. Capture: HA723440, Mouse Factor XII Rabbit mAb [PSH12-21] Detector: HA723441, Mouse Factor XII Rabbit mAb [PSH12-22] Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 26,213,785 pg/mL in mouse serum and 24,389 pg/ml in mouse liver Supernatant.

Interpolated concentrations of spiked Factor XII in cell culture media samples. Capture: HA723440, Mouse Factor XII Rabbit mAb [PSH12-21] Detector: HA723441, Mouse Factor XII Rabbit mAb [PSH12-22] The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse Factor XII
HUABIO
catalog: HA723441

domestic rabbit monoclonal (PSH12-22)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse Factor XII matched pair antibodies Capture: HA723440, Mouse Factor XII Rabbit mAb [PSH12-21] Detector: HA723441, Mouse Factor XII Rabbit mAb [PSH12-22] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723440) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Factor XII protein (HA210984) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA723441, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Factor XII in mouse samples. Capture: HA723440, Mouse Factor XII Rabbit mAb [PSH12-21] Detector: HA723441, Mouse Factor XII Rabbit mAb [PSH12-22] Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 26,213,785 pg/mL in mouse serum and 24,389 pg/ml in mouse liver Supernatant.

Interpolated concentrations of spiked Factor XII in cell culture media samples. Capture: HA723440, Mouse Factor XII Rabbit mAb [PSH12-21] Detector: HA723441, Mouse Factor XII Rabbit mAb [PSH12-22] The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse Factor XII
HUABIO
catalog: HA723442B

domestic rabbit monoclonal (PSH12-22)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse Factor XII matched pair antibodies Capture: HA723440, Mouse Factor XII Rabbit mAb [PSH12-21] Detector: HA723441, Mouse Factor XII Rabbit mAb [PSH12-22] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723440) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Factor XII protein (HA210984) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA723441, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Factor XII in mouse samples. Capture: HA723440, Mouse Factor XII Rabbit mAb [PSH12-21] Detector: HA723441, Mouse Factor XII Rabbit mAb [PSH12-22] Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 26,213,785 pg/mL in mouse serum and 24,389 pg/ml in mouse liver Supernatant.

Interpolated concentrations of spiked Factor XII in cell culture media samples. Capture: HA723440, Mouse Factor XII Rabbit mAb [PSH12-21] Detector: HA723441, Mouse Factor XII Rabbit mAb [PSH12-22] The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier

Mouse/Rat Factor XII
HUABIO
catalog: HA723443

domestic rabbit monoclonal (PSH12-21)
reactivity: mouse, rat
application: ELISA

Sandwich ELISA analysis of Mouse Factor XII matched pair antibodies Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723443) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Factor XII protein (HA210984) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA723444, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Factor XII in mouse samples. Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 22,555,940 pg/mL in mouse serum and 20,935 pg/ml in mouse liver Supernatant.

Interpolated concentrations of spiked Factor XII in cell culture media samples. Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse/Rat Factor XII
HUABIO
catalog: HA723444

domestic rabbit monoclonal (PSH12-23)
reactivity: mouse, rat
application: ELISA

Sandwich ELISA analysis of Mouse Factor XII matched pair antibodies Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723443) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Factor XII protein (HA210984) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA723444, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Factor XII in mouse samples. Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 22,555,940 pg/mL in mouse serum and 20,935 pg/ml in mouse liver Supernatant.

Interpolated concentrations of spiked Factor XII in cell culture media samples. Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse/Rat Factor XII
HUABIO
catalog: HA723445B

domestic rabbit monoclonal (PSH12-23)
reactivity: mouse, rat
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse Factor XII matched pair antibodies Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723443) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Factor XII protein (HA210984) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA723444, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Factor XII in mouse samples. Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 22,555,940 pg/mL in mouse serum and 20,935 pg/ml in mouse liver Supernatant.

Interpolated concentrations of spiked Factor XII in cell culture media samples. Capture: HA723443, Mouse/Rat Factor XII Rabbit mAb [PSH12-21] Detector: HA723444, Mouse/Rat Factor XII Rabbit mAb [PSH12-23] The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier

Mouse Factor XII
HUABIO
catalog: HA723509H

domestic rabbit monoclonal (PSH12-22)
reactivity: mouse
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of Mouse Factor XII matched pair antibodies Capture: HA723440, Mouse Factor XII Rabbit mAb [PSH12-21] Detector: HA723441, Mouse Factor XII Rabbit mAb [PSH12-22] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723440) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Factor XII protein (HA210984) starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA723441, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier