Nectin 2
HUABIO
catalog: ET7108-95
domestic rabbit monoclonal (JG39-64)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Nectin 2 on different lysates with Rabbit anti-Nectin 2 antibody (ET7108-95) at 1/5,000 dilution. Lane 1: HT-29 cell lysate Lane 2: A549 cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: Mouse testis tissue lysate Lane 6: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 75 kDa Exposure time: 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-95) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Nectin 2 on different lysates with Rabbit anti-Nectin 2 antibody (ET7108-95) at 1/10,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Nectin 2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 58 kDa Observed band size: 70-85 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-95) at 1/10,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of MCF7 cells labeling Nectin 2 with Rabbit anti-Nectin 2 antibody (ET7108-95) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Nectin 2 antibody (ET7108-95) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
Nectin 2
HUABIO
catalog: HA723346
domestic rabbit monoclonal (PSH11-54)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of Nectin 2 on different lysates with Rabbit anti-Nectin 2 antibody (HA723346) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MOLT-4 cell lysate (negative) Lane 3: HT-29 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 58-75 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723346) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of MCF7 (positive) and MOLT-4 (negative) labeling Nectin 2 with Rabbit anti-Nectin 2 antibody (HA723346) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Nectin 2 antibody (HA723346) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of MOLT-4 (left, negative) and MCF7 (right, positive) cells labeling Nectin 2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723346, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
Human Nectin-2
HUABIO
catalog: HA723460
domestic rabbit monoclonal (PSH11-54)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human Nectin-2 matched pair antibodies Capture: HA723460, Human Nectin-2 Rabbit mAb [PSH11-54] Detector: HA723461, Human Nectin-2 Rabbit mAb [PSH12-52] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723460) diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Nectin-2 protein (HA210905) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723461, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Nectin-2 in MCF7 and MOLT-4 extract samples based on a 1,000 µg/ml extract load. Capture: HA723460, Human Nectin-2 Rabbit mAb [PSH11-54] Detector: HA723461, Human Nectin-2 Rabbit mAb [PSH12-52] The concentrations of Nectin-2 were measured in duplicates, interpolated from the Nectin-2 standard curve and corrected for sample dilution. Undiluted samples are MOLT-4 extract 50% and MCF7 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nectin-2 concentration was determined to be 3,548 pg/ml in MCF7 extract and undetectable in MOLT-4 extract.

Interpolated concentrations of spiked Nectin-2 in human cell culture media samples. Capture: HA723460, Human Nectin-2 Rabbit mAb [PSH11-54] Detector: HA723461, Human Nectin-2 Rabbit mAb [PSH12-52] The concentrations of Nectin-2 were measured in duplicates, interpolated from the Nectin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human Nectin-2
HUABIO
catalog: HA723461
domestic rabbit monoclonal (PSH12-52)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human Nectin-2 matched pair antibodies Capture: HA723460, Human Nectin-2 Rabbit mAb [PSH11-54] Detector: HA723461, Human Nectin-2 Rabbit mAb [PSH12-52] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723460) diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Nectin-2 protein (HA210905) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723461, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of human Nectin-2 matched pair antibodies Capture: HA723463, Human Nectin-2 Rabbit mAb [PSH12-53] Detector: HA723461, Human Nectin-2 Rabbit mAb [PSH12-52] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723463) diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Nectin-2 protein (HA210905) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723461, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Nectin-2 in MCF7 and MOLT-4 extract samples based on a 1,000 µg/ml extract load. Capture: HA723460, Human Nectin-2 Rabbit mAb [PSH11-54] Detector: HA723461, Human Nectin-2 Rabbit mAb [PSH12-52] The concentrations of Nectin-2 were measured in duplicates, interpolated from the Nectin-2 standard curve and corrected for sample dilution. Undiluted samples are MOLT-4 extract 50% and MCF7 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nectin-2 concentration was determined to be 3,548 pg/ml in MCF7 extract and undetectable in MOLT-4 extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Human Nectin-2
HUABIO
catalog: HA723462B
domestic rabbit monoclonal (PSH12-52)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human Nectin-2 matched pair antibodies Capture: HA723460, Human Nectin-2 Rabbit mAb [PSH11-54] Detector: HA723461, Human Nectin-2 Rabbit mAb [PSH12-52] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723460) diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Nectin-2 protein (HA210905) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723461, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of human Nectin-2 matched pair antibodies Capture: HA723463, Human Nectin-2 Rabbit mAb [PSH12-53] Detector: HA723461, Human Nectin-2 Rabbit mAb [PSH12-52] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723463) diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Nectin-2 protein (HA210905) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723461, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Nectin-2 in MCF7 and MOLT-4 extract samples based on a 1,000 µg/ml extract load. Capture: HA723460, Human Nectin-2 Rabbit mAb [PSH11-54] Detector: HA723461, Human Nectin-2 Rabbit mAb [PSH12-52] The concentrations of Nectin-2 were measured in duplicates, interpolated from the Nectin-2 standard curve and corrected for sample dilution. Undiluted samples are MOLT-4 extract 50% and MCF7 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nectin-2 concentration was determined to be 3,548 pg/ml in MCF7 extract and undetectable in MOLT-4 extract.
quantity: 100μl
price: 409.00 USD
to the supplier
Human Nectin-2
HUABIO
catalog: HA723463
domestic rabbit monoclonal (PSH12-53)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human Nectin-2 matched pair antibodies Capture: HA723463, Human Nectin-2 Rabbit mAb [PSH12-53] Detector: HA723461, Human Nectin-2 Rabbit mAb [PSH12-52] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723463) diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Nectin-2 protein (HA210905) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723461, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Nectin-2 in MCF7 and MOLT-4 extract samples based on a 1,000 µg/ml extract load. Capture: HA723463, Human Nectin-2 Rabbit mAb [PSH12-53] Detector: HA723461, Human Nectin-2 Rabbit mAb [PSH12-52] The concentrations of Nectin-2 were measured in duplicates, interpolated from the Nectin-2 standard curve and corrected for sample dilution. Undiluted samples are MOLT-4 extract 50% and MCF7 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nectin-2 concentration was determined to be 4,062 pg/ml in MCF7 extract and undetectable in MOLT-4 extract.

Interpolated concentrations of spiked Nectin-2 in human cell culture media samples. Capture: HA723463, Human Nectin-2 Rabbit mAb [PSH12-53] Detector: HA723461, Human Nectin-2 Rabbit mAb [PSH12-52] The concentrations of Nectin-2 were measured in duplicates, interpolated from the Nectin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Nectin 2
HUABIO
catalog: HA751416
domestic rabbit monoclonal (PSH11-54)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of Nectin 2 on different lysates with Rabbit anti-Nectin 2 antibody (HA751416) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MOLT-4 cell lysate (negative) Lane 3: HT-29 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 58-75 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751416) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of MCF7 (positive) and MOLT-4 (negative) labeling Nectin 2 with Rabbit anti-Nectin 2 antibody (HA751416) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Nectin 2 antibody (HA751416) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of MOLT-4 (left, negative) and MCF7 (right, positive) cells labeling Nectin 2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751416, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier