domestic rabbit monoclonal (SR46-04)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of FAK on different lysates with Rabbit anti-FAK antibody (ET1602-25) at 1/5,000 dilution. Lane 1: A431 cell lysate (15 µg/Lane) Lane 2: U-87 MG cell lysate (15 µg/Lane) Lane 3: NIH/3T3 cell lysate (15 µg/Lane) Lane 4: C2C12 cell lysate (15 µg/Lane) Lane 5: C6 cell lysate (15 µg/Lane) Predicted band size: 119 kDa Observed band size: 119 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-25) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of FAK on different lysates with Rabbit anti-FAK antibody (ET1602-25) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate (10 µg/Lane) Lane 2: Hela-si FAK#1 cell lysate (10 µg/Lane) Lane 3: Hela-si FAK#2 cell lysate (10 µg/Lane) Predicted band size: 119 kDa Observed band size: 119 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. ET1602-25 was shown to specifically react with FAK in Hela-si NT cells. No bands were observed when Hela-si FAK samples were tested. Hela-si NT and Hela-si FAK samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1602-25, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
FAK was immunoprecipitated in 0.2mg A431 cell lysate with ET1602-25 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1602-25 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input) Lane 2: ET1602-25 IP in A431 cell lysate Lane 3: Rabbit IgG instead of ET1602-25 in A431 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (SC54-07)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Phospho-FAK (Y397) on different lysates with Rabbit anti-Phospho-FAK (Y397) antibody (ET1610-34) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: PC-3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 119 kDa Observed band size: 119 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-34) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-FAK (Y397) on Hela cell lysates. Lane 1: Hela cells, whole cell lysate, 10ug/lane Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-FAK (Y397) antibody (ET1610-34) at 1:500 dilution. Anti-Hsp90 antibody (ET1605-56) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 119 kDa Observed band size: 119 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 30 seconds
Immunocytochemistry analysis of NIH/3T3 cells labeling Phospho-FAK (Y397) with Rabbit anti-Phospho-FAK (Y397) antibody (ET1610-34) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-FAK (Y397) antibody (ET1610-34) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (SR46-04)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of FAK on different lysates with Rabbit anti-FAK antibody (HA750039) at 1/5,000 dilution. Lane 1: A431 cell lysate (15 µg/Lane) Lane 2: U-87 MG cell lysate (15 µg/Lane) Lane 3: NIH/3T3 cell lysate (15 µg/Lane) Lane 4: C2C12 cell lysate (15 µg/Lane) Lane 5: C6 cell lysate (15 µg/Lane) Predicted band size: 119 kDa Observed band size: 119 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750039) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of FAK on different lysates with Rabbit anti-FAK antibody (HA750039) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate (10 µg/Lane) Lane 2: Hela-si FAK#1 cell lysate (10 µg/Lane) Lane 3: Hela-si FAK#2 cell lysate (10 µg/Lane) Predicted band size: 119 kDa Observed band size: 119 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. ET1602-25 was shown to specifically react with FAK in Hela-si NT cells. No bands were observed when Hela-si FAK samples were tested. Hela-si NT and Hela-si FAK samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1602-25, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
FAK was immunoprecipitated in 0.2mg A431 cell lysate with HA750039 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750039 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input) Lane 2: HA750039 IP in A431 cell lysate Lane 3: Rabbit IgG instead of HA750039 in A431 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (SC54-07)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Phospho-FAK (Y397) on different lysates with Rabbit anti-Phospho-FAK (Y397) antibody (HA750212) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: PC-3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 119 kDa Observed band size: 119 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750212) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-FAK (Y397) on Hela cell lysates. Lane 1: Hela cells, whole cell lysate, 10ug/lane Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-FAK (Y397) antibody (HA750212) at 1:500 dilution. Anti-Hsp90 antibody (ET1605-56) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 119 kDa Observed band size: 119 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 30 seconds
Immunocytochemistry analysis of NIH/3T3 cells labeling Phospho-FAK (Y397) with Rabbit anti-Phospho-FAK (Y397) antibody (HA750212) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-FAK (Y397) antibody (HA750212) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier