domestic rabbit monoclonal (PSH16-03)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of Mouse Resistin matched pair antibodies Capture: HA723808, Mouse Resistin Rabbit mAb [PSH16-03] Detector: HA723809, Mouse Resistin Rabbit mAb [PSH16-04] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723808) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Resistin protein (HA211042) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723809, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Resistin in Mouse serum. Capture: HA723808, Mouse Resistin Rabbit mAb [PSH16-03] Detector: HA723809, Mouse Resistin Rabbit mAb [PSH16-04] Interpolated concentration of native Resistin was measured in duplicate at different sample concentrations and interpolated from the RETN standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Resistin concentration was determined to be 44636 pg/ml in Mouse serum.
Interpolated concentrations of spiked Resistin in cell culture media samples. Capture: HA723808, Mouse Resistin Rabbit mAb [PSH16-03] Detector: HA723809, Mouse Resistin Rabbit mAb [PSH16-04] The concentrations of Resistin were measured in duplicates, interpolated from the Resistin standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-04)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of Mouse Resistin matched pair antibodies Capture: HA723808, Mouse Resistin Rabbit mAb [PSH16-03] Detector: HA723809, Mouse Resistin Rabbit mAb [PSH16-04] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723808) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Resistin protein (HA211042) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723809, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Resistin in Mouse serum. Capture: HA723808, Mouse Resistin Rabbit mAb [PSH16-03] Detector: HA723809, Mouse Resistin Rabbit mAb [PSH16-04] Interpolated concentration of native Resistin was measured in duplicate at different sample concentrations and interpolated from the RETN standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Resistin concentration was determined to be 44636 pg/ml in Mouse serum.
Interpolated concentrations of spiked Resistin in cell culture media samples. Capture: HA723808, Mouse Resistin Rabbit mAb [PSH16-03] Detector: HA723809, Mouse Resistin Rabbit mAb [PSH16-04] The concentrations of Resistin were measured in duplicates, interpolated from the Resistin standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-04)
reactivity: mouse
conjugate: biotin
application: ELISA
reactivity: mouse
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Mouse Resistin matched pair antibodies Capture: HA723808, Mouse Resistin Rabbit mAb [PSH16-03] Detector: HA723809, Mouse Resistin Rabbit mAb [PSH16-04] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723808) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Resistin protein (HA211042) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723809, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Resistin in Mouse serum. Capture: HA723808, Mouse Resistin Rabbit mAb [PSH16-03] Detector: HA723809, Mouse Resistin Rabbit mAb [PSH16-04] Interpolated concentration of native Resistin was measured in duplicate at different sample concentrations and interpolated from the RETN standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Resistin concentration was determined to be 44636 pg/ml in Mouse serum.
Interpolated concentrations of spiked Resistin in cell culture media samples. Capture: HA723808, Mouse Resistin Rabbit mAb [PSH16-03] Detector: HA723809, Mouse Resistin Rabbit mAb [PSH16-04] The concentrations of Resistin were measured in duplicates, interpolated from the Resistin standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-04)
reactivity: mouse
conjugate: HRP
application: ELISA
reactivity: mouse
conjugate: HRP
application: ELISA
Sandwich ELISA analysis of Mouse Resistin matched pair antibodies Capture: HA723808, Mouse Resistin Rabbit mAb [PSH16-03] Detector: HA723809, Mouse Resistin Rabbit mAb [PSH16-04] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723808) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Resistin protein (HA211042) starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA723809, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier