domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of VGLUT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500083, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human brain tissue lysate Lane 2: Mouse hippocampus tissue lysate
Western blot analysis of VGLUT1 on rat brain tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500083, 1/200,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-VGLUT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500083, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of VGluT1 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500488, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Western blot analysis of VGluT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500488, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse cerebellum tissue lysate
quantity: 100μl
price: 330 USD
to the supplier
mouse monoclonal (A7A3)
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of VGLUT1 on different lysates with Mouse anti-VGLUT1 antibody (HA601014) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Human brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 52 kDa Exposure time: 1 minute 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601014) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier
domestic rabbit monoclonal (PSH10-53)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
Application: IHC-Fr Species: Rat Site: Hippocampus Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH10-53)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
Application: IHC-Fr Species: Rat Site: Hippocampus Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
quantity: 100μl
price: 649.00 USD
to the supplier