HUABIO MAPK10 antibody

JNK1+JNK2+JNK3
HUABIO
catalog: ET1601-28

domestic rabbit monoclonal (SA43-06)
reactivity: human, mouse, rat, bovine, zebrafish
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of JNK1+JNK2+JNK3 on different lysates with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 35 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-28) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of JNK1+JNK2+JNK3 on different lysates with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (15 µg/Lane) Lane 2: Rat brain tissue lysate (15 µg/Lane) Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-28) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-28) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-JNK1/2/3 (T183 + T183 + T221)
HUABIO
catalog: ET1609-42

domestic rabbit monoclonal (ST500)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of Phospho-JNK1/2/3 (T183 + T183 + T221) on different lysates with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (ET1609-42) at 1/1,000 dilution. Lane 1: Hela cell lysate, untreated (10 µg/Lane) Lane 2: Hela cell lysate, treated with Anisomycin (10 µg/Lane) Lane 3: A431 cell lysate, untreated (10 µg/Lane) Lane 4: A431 cell lysate, treated with UV40 (10 µg/Lane) Lane 5: 293 cell lysate, untreated (10 µg/Lane) Lane 6: 293 cell lysate, treated with UV40 (10 µg/Lane) Lane 7: Hela cell lysate, untreated (10 µg/Lane) Lane 8: Hela cell lysate, treated with UV40 (10 µg/Lane) Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-42) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Phospho-JNK1/2/3 (T183 + T183 + T221) on different lysates with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (ET1609-42) at 1/2,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 25µg/mL Anisomycin for 30 minutes cell lysate. Lysates/proteins at 20 µg/Lane. Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 2 minutes 6 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-42) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Phospho-JNK1/2/3 (T183 + T183 + T221) on different lysates with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (ET1609-42) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 25μg/mL Anisomycin for 30 minutes cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-42) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

JNK3
HUABIO
catalog: ET1612-68

domestic rabbit monoclonal (SD082-09)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of JNK3 on different lysates with Rabbit anti-JNK3 antibody (ET1612-68) at 1/5,000 dilution. Lane 1: MCF7 cell lysate (10 µg/Lane) Lane 2: Mouse brain tissue lysate (20 µg/Lane) Lane 3: Mouse testis tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Lane 5: Rat testis tissue lysate (20 µg/Lane) Predicted band size: 53 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-68) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-JNK3 antibody (ET1612-68) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-68) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-JNK3 antibody (ET1612-68) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-68) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

JNK1+JNK3
HUABIO
catalog: ET1701-47

domestic rabbit monoclonal (JJ08-86)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of JNK1+JNK3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: PC-12 cell lysate

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-JNK1+JNK3 antibody (ET1701-47) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-47) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

JNK1+JNK2+JNK3
HUABIO
catalog: HA750018

domestic rabbit monoclonal (SA43-06)
reactivity: human, mouse, rat, bovine, zebrafish
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of JNK1+JNK2+JNK3 on different lysates with Rabbit anti-JNK1+JNK2+JNK3 antibody (HA750018) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 35 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750018) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of JNK1+JNK2+JNK3 on different lysates with Rabbit anti-JNK1+JNK2+JNK3 antibody (HA750018) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (15 µg/Lane) Lane 2: Rat brain tissue lysate (15 µg/Lane) Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750018) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-JNK1+JNK2+JNK3 antibody (HA750018) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750018) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-JNK1/2/3 (T183 + T183 + T221)
HUABIO
catalog: HA750173

Western blot analysis of Phospho-JNK1/2/3 (T183 + T183 + T221) on different lysates with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (HA750173) at 1/1,000 dilution. Lane 1: Hela cell lysate, untreated (10 µg/Lane) Lane 2: Hela cell lysate, treated with Anisomycin (10 µg/Lane) Lane 3: A431 cell lysate, untreated (10 µg/Lane) Lane 4: A431 cell lysate, treated with UV40 (10 µg/Lane) Lane 5: 293 cell lysate, untreated (10 µg/Lane) Lane 6: 293 cell lysate, treated with UV40 (10 µg/Lane) Lane 7: Hela cell lysate, untreated (10 µg/Lane) Lane 8: Hela cell lysate, treated with UV40 (10 µg/Lane) Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750173) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Phospho-JNK1/2/3 (T183 + T183 + T221) on different lysates with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (HA750173) at 1/2,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 25µg/mL Anisomycin for 30 minutes cell lysate. Lysates/proteins at 20 µg/Lane. Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 2 minutes 6 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750173) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Phospho-JNK1/2/3 (T183 + T183 + T221) on different lysates with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (HA750173) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 25μg/mL Anisomycin for 30 minutes cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750173) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

Anti-JNK1+JNK2+JNK3 Antibody [SA43-06]
HUABIO
catalog: ET1601-28TR

domestic rabbit monoclonal (SA43-06)
reactivity: human, mouse, rat, bovine, zebrafish
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 20 uL
price: 99 USD
to the supplier

Anti-Phospho-JNK1/2/3(T183+T183+T221) Antibody [ST500]
HUABIO
catalog: ET1609-42TR

quantity: 20 uL
price: 99 USD
to the supplier