domestic rabbit monoclonal (PSH03-59)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of p38 beta/MAPK11 + p38 gamma/MAPK12 + p38 delta/MAPK13 on different lysates with Rabbit anti-p38 beta/MAPK11 + p38 gamma/MAPK12 + p38 delta/MAPK13 antibody (HA722018) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: HeLa cell lysate (30 µg/Lane) Lane 3: MCF7 cell lysate (30 µg/Lane) Lane 4: K-562 cell lysate (30 µg/Lane) Lane 5: MDA-MB-231 cell lysate (30 µg/Lane) Lane 6: COS-1 cell lysate (30 µg/Lane) Lane 7: RAW264.7 cell lysate (30 µg/Lane) Lane 8: Neuro-2a cell lysate (30 µg/Lane) Lane 9: PC-12 cell lysate (30 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 1 minute 2 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722018) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of p38 beta/MAPK11 + p38 gamma/MAPK12 + p38 delta/MAPK13 with Rabbit anti-p38 beta/MAPK11 + p38 gamma/MAPK12 + p38 delta/MAPK13 antibody (HA722018) at 1/2,000 dilution. Lane 1: Human p38 gamma recombinant protein, 30ng/Lane Lane 2: Human p38 alpha recombinant protein, 50ng/Lane Lane 3: Human p38 delta recombinant protein, 50ng/Lane Lane 4: Human p38 beta recombinant protein, 50ng/Lane Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722018) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH03-60)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 on different lysates with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody (HA722019) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: HeLa cell lysate (30 µg/Lane) Lane 3: K-562 cell lysate (30 µg/Lane) Lane 4: MDA-MB-231 cell lysate (30 µg/Lane) Lane 5: COS-1 cell lysate (30 µg/Lane) Lane 6: Neuro-2a cell lysate (30 µg/Lane) Lane 7: PC-12 cell lysate (30 µg/Lane) Lane 8: Mouse skeletal muscle tissue lysate (30 µg/Lane) Lane 9: Rat skeletal muscle tissue lysate (30 µg/Lane) Lane 10: Rat heart tissue lysate (30 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722019) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody (HA722019) at 1/2,000 dilution. Lane 1: Human p38 gamma recombinant protein, 30ng/Lane Lane 2: Human p38 alpha recombinant protein, 50ng/Lane Lane 3: Human p38 delta recombinant protein, 50ng/Lane Lane 4: Human p38 beta recombinant protein, 50ng/Lane Exposure time: 43 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722019) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH03-59)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of p38 beta/MAPK11 + p38 gamma/MAPK12 + p38 delta/MAPK13 on different lysates with Rabbit anti-p38 beta/MAPK11 + p38 gamma/MAPK12 + p38 delta/MAPK13 antibody (HA750893) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: HeLa cell lysate (30 µg/Lane) Lane 3: MCF7 cell lysate (30 µg/Lane) Lane 4: K-562 cell lysate (30 µg/Lane) Lane 5: MDA-MB-231 cell lysate (30 µg/Lane) Lane 6: COS-1 cell lysate (30 µg/Lane) Lane 7: RAW264.7 cell lysate (30 µg/Lane) Lane 8: Neuro-2a cell lysate (30 µg/Lane) Lane 9: PC-12 cell lysate (30 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 1 minute 2 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750893) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of p38 beta/MAPK11 + p38 gamma/MAPK12 + p38 delta/MAPK13 with Rabbit anti-p38 beta/MAPK11 + p38 gamma/MAPK12 + p38 delta/MAPK13 antibody (HA750893) at 1/2,000 dilution. Lane 1: Human p38 gamma recombinant protein, 30ng/Lane Lane 2: Human p38 alpha recombinant protein, 50ng/Lane Lane 3: Human p38 delta recombinant protein, 50ng/Lane Lane 4: Human p38 beta recombinant protein, 50ng/Lane Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750893) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH03-60)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 on different lysates with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody (HA750894) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: HeLa cell lysate (30 µg/Lane) Lane 3: K-562 cell lysate (30 µg/Lane) Lane 4: MDA-MB-231 cell lysate (30 µg/Lane) Lane 5: COS-1 cell lysate (30 µg/Lane) Lane 6: Neuro-2a cell lysate (30 µg/Lane) Lane 7: PC-12 cell lysate (30 µg/Lane) Lane 8: Mouse skeletal muscle tissue lysate (30 µg/Lane) Lane 9: Rat skeletal muscle tissue lysate (30 µg/Lane) Lane 10: Rat heart tissue lysate (30 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750894) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody (HA750894) at 1/2,000 dilution. Lane 1: Human p38 gamma recombinant protein, 30ng/Lane Lane 2: Human p38 alpha recombinant protein, 50ng/Lane Lane 3: Human p38 delta recombinant protein, 50ng/Lane Lane 4: Human p38 beta recombinant protein, 50ng/Lane Exposure time: 43 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750894) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier