DFNA5 / GSDME
HUABIO
catalog: HA722836

domestic rabbit monoclonal (PSH07-48)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA722836) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: NCI-H226 cell lysate Lane 3: A549 cell lysate Lane 4: C2C12 cell lysate Lane 5: Neuro-2a cell lysate Lane 6: Human brain tissue lysate Lane 7: Mouse brain tissue lysate Lane 8: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722836) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

DFNA5 / GSDME
HUABIO
catalog: HA723113

domestic rabbit monoclonal (PSH09-51)
reactivity: human, mouse
application: western blot, immunoprecipitation

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA723113) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: SW480 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55/25 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723113) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA723113) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with mouse GSDME (C-terminal) cell lysate Lane 3: 293T transfected with mouse GSDME (N-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 51 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723113) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

DFNA5 / GSDME was immunoprecipitated from 0.2 mg SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate with HA723113 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723113 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate (input) Lane 2: HA723113 IP in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: Rabbit IgG instead of HA723113 in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801
quantity: 100μl
price: 385.00 USD
to the supplier

DFNA5 / GSDME
HUABIO
catalog: HA723251

domestic rabbit monoclonal (PSH10-84)
reactivity: human, mouse
application: western blot, immunoprecipitation

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA723251) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: SW480 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55/30 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723251) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA723251) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with mouse GSDME (N-terminal) cell lysate Lane 3: 293T transfected with mouse GSDME (C-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723251) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

DFNA5 / GSDME was immunoprecipitated from 0.2 mg SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate with HA723251 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723251 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate (input) Lane 2: HA723251 IP in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: Rabbit IgG instead of HA723251 in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801
quantity: 100μl
price: 385.00 USD
to the supplier

DFNA5 / GSDME
HUABIO
catalog: HA751169

domestic rabbit monoclonal (PSH07-48)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA751169) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: NCI-H226 cell lysate Lane 3: A549 cell lysate Lane 4: C2C12 cell lysate Lane 5: Neuro-2a cell lysate Lane 6: Human brain tissue lysate Lane 7: Mouse brain tissue lysate Lane 8: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751169) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

DFNA5 / GSDME
HUABIO
catalog: HA751298

domestic rabbit monoclonal (PSH09-51)
reactivity: human, mouse
application: western blot, immunoprecipitation

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA751298) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: SW480 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55/25 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751298) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA751298) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with mouse GSDME (C-terminal) cell lysate Lane 3: 293T transfected with mouse GSDME (N-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 51 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751298) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

DFNA5 / GSDME was immunoprecipitated from 0.2 mg SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate with HA751298 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751298 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate (input) Lane 2: HA751298 IP in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: Rabbit IgG instead of HA751298 in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801
quantity: 100μl
price: 649.00 USD
to the supplier

DFNA5 / GSDME
HUABIO
catalog: HA751376

domestic rabbit monoclonal (PSH10-84)
reactivity: human, mouse
application: western blot, immunoprecipitation

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA751376) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: SW480 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55/30 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751376) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA751376) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with mouse GSDME (N-terminal) cell lysate Lane 3: 293T transfected with mouse GSDME (C-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751376) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

DFNA5 / GSDME was immunoprecipitated from 0.2 mg SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate with HA751376 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751376 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate (input) Lane 2: HA751376 IP in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: Rabbit IgG instead of HA751376 in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801
quantity: 100μl
price: 649.00 USD
to the supplier