mouse monoclonal (A3-C11)
reactivity: human, mouse
application: western blot, flow cytometry
reactivity: human, mouse
application: western blot, flow cytometry
Western blot analysis of Oct4 on different lysates with Mouse anti-Oct4 antibody (EM100306) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Oct4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 39 kDa Observed band size: 42 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM100306) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Oct4 on different lysates using anti-Oct4 antibody at 1/1,000 dilution. Positive control: Lane 1: NCCIT cell lysate Lane 2: D3 cell lysate
ICC staining of OCT-4 in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 360.00 USD
to the supplier
domestic rabbit monoclonal (SD0750)
reactivity: human, mouse
application: western blot, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section
reactivity: human, mouse
application: western blot, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section
Immunocytochemistry analysis of NCCIT cells labeling Oct4 with Rabbit anti-Oct4 antibody (ET1612-20) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Oct4 antibody (ET1612-20) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Immunocytochemistry analysis of F9 cells labeling Oct4 with Rabbit anti-Oct4 antibody (ET1612-20) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Oct4 antibody (ET1612-20) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Western blot analysis of Oct4 with anti-Oct4 antibody (ET1612-20) at 1/2,000 dilution. Lane 1: Wild-type NCCIT whole cell lysate. Lane 2: Oct4 knockout NCCIT whole cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Oct4 antibody (ET1612-20, 1/2,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
mouse monoclonal (A3-C11-R)
reactivity: human
application: western blot
reactivity: human
application: western blot
Western blot analysis of Oct4 on NCCIT cell lysates with Mouse anti-Oct4 antibody (HA601207) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 39 kDa Observed band size: 45 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601207) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Oct4 on different lysates with Mouse anti-Oct4 antibody (HA601207) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Oct4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 39 kDa Observed band size: 42 kDa Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601207) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of NCCIT cells labeling Oct4 with Mouse anti-Oct4 antibody (HA601207) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Oct4 antibody (HA601207) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 360.00 USD
to the supplier
mouse monoclonal (A3-C11-R)
reactivity: human
application: western blot
reactivity: human
application: western blot
Western blot analysis of Oct4 on NCCIT cell lysates with Mouse anti-Oct4 antibody (HA610085) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 39 kDa Observed band size: 45 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610085) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Oct4 on different lysates with Mouse anti-Oct4 antibody (HA610085) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Oct4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 39 kDa Observed band size: 42 kDa Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610085) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of NCCIT cells labeling Oct4 with Mouse anti-Oct4 antibody (HA610085) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Oct4 antibody (HA610085) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
quantity: 100μg
price: 649.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
